The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene

HA Shi-A-Gu-La; SHI Yi-Shen; Zhang He-Ling

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1992

HA Shi-A-Gu-La, SHI Yi-Shen and Zhang He-Ling. The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene[J]. Virologica Sinica, 1992, 7(4).

Citation: HA Shi-A-Gu-La, SHI Yi-Shen, Zhang He-Ling. The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene .VIROLOGICA SINICA, 1992, 7(4) : 432.

马铃薯卷叶病毒外壳蛋白基因的合成、分子克隆和全序列分析

1.
内蒙古大学生物系
2.
内蒙古大学生物系呼和浩特
3.
呼和浩特

摘要

以分离自马铃薯主栽品种“紫花白”的马铃薯卷叶病毒(PLRV)分离物的RNA为模板,用人工合成的引物,用反转录和随后PCR扩增的方法合成了PLRV外壳蛋白(CP)基因的cDNA,并克隆于pUC19中。进一步用限制酶切和核苷酸序列分析表明,合成的cDNA由627个核苷酸组成(包括起始和终止密码),序列中有Hinc Ⅱ和BamH Ⅰ两个酶切位点,与国外报道一致。和国外的4个PLRV分离物CP基因序列对比结果,具有高度同源性,其同源率达99.0—99.7％。
- 马铃薯卷叶病毒（PLRV）
- , 外壳蛋白基因
- , 分子克隆
- , 序列分析
- , PCR

The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene

1.
Department of Biology
2.
Inner Mongolia University
3.
lluhehaotc

Abstract

A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. The synthesized eDNA was cloned in plasmid pUC19 in JM103. The full length CP gene eDNA clones pLCP2 and pLCP4 were further identified by restriction mapping analysis and, the nucleotide sequence of cDNA cloned in pLCP2 was analyzed with ABI 370 DNA sequence Tests show that th...
- Potato leaf roll virus（PLRV）
- , Coat protein
- , gene Molecular
- , cloning Sequence analysis PCR

References
Proportional views

PDF

Article Metrics

Article views(3746) PDF downloads(928) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene

1. Department of Biology
2. Inner Mongolia University
3. lluhehaotc

Abstract: A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. The synthesized eDNA was cloned in plasmid pUC19 in JM103. The full length CP gene eDNA clones pLCP2 and pLCP4 were further identified by restriction mapping analysis and, the nucleotide sequence of cDNA cloned in pLCP2 was analyzed with ABI 370 DNA sequence Tests show that th...