Cleavage of the Spike Protein and Cytopathogenesis of Avian Infectious Bronchitis Virus

LI De-Shan; D.Cavanagh

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August 1992

LI De-Shan and D.Cavanagh. Cleavage of the Spike Protein and Cytopathogenesis of Avian Infectious Bronchitis Virus[J]. Virologica Sinica, 1992, 7(4).

Citation: LI De-Shan, D.Cavanagh. Cleavage of the Spike Protein and Cytopathogenesis of Avian Infectious Bronchitis Virus .VIROLOGICA SINICA, 1992, 7(4) : 436.

鸡传染性支气管炎病毒纤突蛋白裂解与致病性的研究

李德山 ,
D.Cavanagh

1.
中国农业科学院哈尔滨兽医研究所
2.
英国豪顿禽病研究所哈尔滨

摘要

冠状病毒的致病机理一直不清楚。对副粘病毒和正粘病毒的研究结果表明病毒的致病力与病毒纤突蛋白裂解程度有关,反过来纤突蛋白裂解程度是由纤突蛋白的连结多肽的氨基酸顺序决定的。本文试图从IBV致细胞病变作用,纤突蛋白裂解状态和连结多肽氨基酸顺序三个方面探讨IBV的致病机理。结果表明,IBV毒株在不同细胞培养(CK.CEF和Vero)中,从宿主细胞范围、致细胞病变作用和引起细胞融合几项指标表现了不同的致病力,但不同毒株从同一种细胞释放后其纤突蛋白的裂解程度无差异。连结多肽的氮基酸序列表明23株IBV的连结多肽均由5个氨基酸组成即二对碱性氨基酸Arg—Arg和Arg—Arg,中间由苯丙氨酸或丝氨酸联接。这些结果说明在致病机理方面,冠状病毒可能不同于副粘病毒和正粘病毒。
- 冠状病毒
- , IBV
- , 纤突蛋白
- , 连结多肽
- , 裂解

Cleavage of the Spike Protein and Cytopathogenesis of Avian Infectious Bronchitis Virus

LI De-Shan ,
D.Cavanagh

1.
Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001Houghton Poultry Research Station
2.
U.K

Abstract

Studies on paramyxoviruses and myxoviruses have shown that the viral pathogenesis is related to the cleavibility of the viral spike protein, in turn, cleavibility of the spike protein is determined by the amino acid sequence of the connecting peptide of the spike protein This paper intended to investigate the pathogenesis of avian coronavirus, infectious bronchitis virus(IBV) by looking into three aspects, i. e. cytopathogenesis of the virus, cleavage status of the viral spike protein and amino acid sequenc...
- Coronavirus
- , IBV
- , Spike protein
- , Connecting peptide
- , Cleavage

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cleavage of the Spike Protein and Cytopathogenesis of Avian Infectious Bronchitis Virus

LI De-Shan ,
D.Cavanagh

1. Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Harbin 150001Houghton Poultry Research Station
2. U.K

Abstract: Studies on paramyxoviruses and myxoviruses have shown that the viral pathogenesis is related to the cleavibility of the viral spike protein, in turn, cleavibility of the spike protein is determined by the amino acid sequence of the connecting peptide of the spike protein This paper intended to investigate the pathogenesis of avian coronavirus, infectious bronchitis virus(IBV) by looking into three aspects, i. e. cytopathogenesis of the virus, cleavage status of the viral spike protein and amino acid sequenc...