Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene

WANG Jin-Fang; QIU Bing-Sheng; TIAN Bo

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August 1992

WANG Jin-Fang, QIU Bing-Sheng and TIAN Bo. Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene[J]. Virologica Sinica, 1992, 7(4).

Citation: WANG Jin-Fang, QIU Bing-Sheng, TIAN Bo. Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene .VIROLOGICA SINICA, 1992, 7(4) : 463.

水稻条纹叶枯病毒分子生物学的研究——Ⅲ.外壳蛋白基因的序列分析

1.
中国科学院微生物所
2.
中国科学院微生物所北京

摘要

以含有水稻条纹叶枯病毒(RSV)外壳蛋白(CP)基因的重组克隆为材料,将RSV-cDNA酶解后,亚克隆人M13mp18、19,采用双脱氧链终止法测序得到RSV-CP基因的全长序列(共含966bp),同日本已发表的RSV-CP基因序列相比同源率达97％。
- 水稻条纹叶枯病毒
- , 外壳蛋白基因
- , DNA序列

Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene

1.
Institute of Microbiology
2.
Academia Sinica

Abstract

We used the recombinant clone eontaing RSV-CP gene as material. After being digested with restriction endonueleases, RSV-eDNA fragments were subcloned into M13 rap18, 19. The CP gene sequence was determined by dideoxynucleotide chain termination method and the result revealed that RSVCP gene consists of 966 base pairs with 97% sequence homology to the Japanese isolate.
- Rice stripe
- , virus Coat protein
- , DNA sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on Molecular Biology of Rice Stripe Virus Ⅲ. Sequence Analysis of Coat Protein Gene

1. Institute of Microbiology
2. Academia Sinica

Abstract: We used the recombinant clone eontaing RSV-CP gene as material. After being digested with restriction endonueleases, RSV-eDNA fragments were subcloned into M13 rap18, 19. The CP gene sequence was determined by dideoxynucleotide chain termination method and the result revealed that RSVCP gene consists of 966 base pairs with 97% sequence homology to the Japanese isolate.