Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments

JIA Dong-Xiang; WANG Mei-Xian; WANG Jing-Yi; XU Zhi-Kai

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February 1993

JIA Dong-Xiang, WANG Mei-Xian, WANG Jing-Yi and XU Zhi-Kai. Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments[J]. Virologica Sinica, 1993, 8(1).

Citation: JIA Dong-Xiang, WANG Mei-Xian, WANG Jing-Yi, XU Zhi-Kai. Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments .VIROLOGICA SINICA, 1993, 8(1) : 53.

肾综合征出血热病毒基因部份酶切片段的亚克隆

1.
军事医学科学院生物工程研究所
2.
第四军医大学分子病毒学研究室
3.
海军总医院分子生物学研究室
4.
第四军医大学分子病毒学研究室北京

摘要

在用微机对肾综合征出血热病毒76/118株(即汉坦病毒)S与M基因片段进行系统分析的基础上,分别利用质粒pXZ62和pUC18亚克隆了S与M基因中的部份酶切片段,以便为亚克隆基因探针的应用和基因改造与多途径表达奠定基础。同时亦进一步证实了XylE基因-邻苯二酚2,3-双加氧酶(CatO2ase)这一显色标记系统在基因工程中的实用价值。
- 出血热病毒
- , 基因
- , 克隆
- , XylE

Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments

1.
The Fourth Military Medical University
2.
Xi an 71OO32Navy General Hospital of PLA
3.
Beijing 100037

Abstract

Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...
- HFRSV
- , Gene clone
- , XylE

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Subcloning of Hemorrhagic Fever with Renal Syndrome Virus (HFRSV) Gene Fragments

1. The Fourth Military Medical University
2. Xi an 71OO32Navy General Hospital of PLA
3. Beijing 100037

Abstract: Based on the systematic analysis of HFRSV strain 76/118 (Hantaan virus) M and S gene with personal computer, the endonucleased fragments of tha two gene cDNA were subcloned for smaller gene probes and expression studies afterwards. The fragment containing G2 gene and the HindⅢ -BamH Ⅰ 437bp fragment of S gene were cloned into pX62, the positive recombinants of the former were selectecd by inactivation of XylE gene, a chromogenic selective marker, while the latter did not inactivate XylE gene expression, in ...