Purification and Serology of Sweet Potato Feathery Mottle Virus

SHU Zuo-Wei; XUE Qi-Han

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February 1993

SHU Zuo-Wei and XUE Qi-Han. Purification and Serology of Sweet Potato Feathery Mottle Virus[J]. Virologica Sinica, 1993, 8(1).

Citation: SHU Zuo-Wei, XUE Qi-Han. Purification and Serology of Sweet Potato Feathery Mottle Virus .VIROLOGICA SINICA, 1993, 8(1) : 84.

甘薯羽状斑驳病毒分离提纯及血清学特性

朱作为 ,
薛启汉

1.
江苏省农科院遗传生理研究所
2.
江苏省农科院遗传生理研究所南京

摘要

从感染有甘薯羽状斑驳病毒的牵牛(Ⅰ.Nil)叶片,通过超速离心,Cscl密度梯度离心提纯SPFMV粒子,每千克叶片可得病毒13—15毫克。电镜观察病毒粒子长度范围在820—860nm,也可见到900nm以上的特长粒子。病毒提纯物的紫外吸收曲线呈典型核蛋白吸收曲线,OD260/OD280=1.21,免疫电镜检查,该病毒与SPFMV抗血清起阳性反应。
- 甘薯羽状斑驳病毒
- , 提纯
- , 免疫电镜

Purification and Serology of Sweet Potato Feathery Mottle Virus

SHU Zuo-Wei ,
XUE Qi-Han

1.
Institute of Genetics
2.
Jiangsu Academy of Agricultural Sciences

Abstract

Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.
- Sweet potato feathery mottle virus
- , Purification
- , Immunosorbent electromicroscopy

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Purification and Serology of Sweet Potato Feathery Mottle Virus

SHU Zuo-Wei ,
XUE Qi-Han

1. Institute of Genetics
2. Jiangsu Academy of Agricultural Sciences

Abstract: Sweet potato feathery mottle virus (SPEMV) was purified from infected I.Nil leaves using ultracentrifugation and CsCl step gradient centrifugations. Virus yields of 13—15 mg Per kilogram of infected Ieaf tissue were obtained. The normal particle lengths of the SPFMV was 820—860nm. The virus obtained was highly infectious and a good immunogen. Purified virus had a OD260/OD280 ratio of 1.21.