Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools

CHEN Wan-Rong; TIAN Xin; BAO Zuo-Yi; YIN Shu-Rong

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February 1993

CHEN Wan-Rong, TIAN Xin, BAO Zuo-Yi and YIN Shu-Rong. Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools[J]. Virologica Sinica, 1993, 8(1).

Citation: CHEN Wan-Rong, TIAN Xin, BAO Zuo-Yi, YIN Shu-Rong. Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools .VIROLOGICA SINICA, 1993, 8(1) : 111.

从实验感染动物粪便中分离出两株疑似戊型肝炎病毒

1.
军事医学科学院微生物流行病研究所
2.
军事医学科学院微生物流行病研究所北京

摘要

用Hep-G_2细胞从肠道传播的非甲非乙型肝炎(ET-NANBH)病人粪便悬液感染恒河猴的潜伏期粪便中分离出两株致细胞病变(CPE)的病毒(R_(19),R_(25))。用免疫电镜(IEM)观察,这两株病毒均为球形,有实心和空心两种颗粒,直径约27—34nm,能被新疆和前苏联ET-NANBH急性期血清特异性凝集,病毒颗粒间抗体桥明显,ET-NANBH急性期血清对这两株病毒亦有一定中和作用,内地甲肝(HA)病人和正常人血清既不能凝集,也不能中和,是否为ET-NANBH病原体尚待进一步鉴定。
- 戊型肝炎病毒
- , 实验动物感染
- , 组织培养
- , 病毒分离

Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools

1.
Institute of Microbiology and Epidemiology
2.
Academy of Military Medical Science

Abstract

The Hep-G_2 cell lines were inoculated for isolating HEV and two strain CPE Viruses (R19, R25)were isolated from experimental infected monkey s acute phase fecal. IEM revealed that the isolated two strain viruses could be aggregated by acute phase sera of ET-NANBH patients from Xinjiang and USSR respectively and there were obvious antibodies and full, spheric 1 27-34nm virus-like empty particles (VLPS). The two strain viruses could be neutralized by acute phase sera of HE patients. The sera of patients from...
- Hepatitis E Virus
- , Experimental infected primates Tissue culture
- , Isolating virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools

1. Institute of Microbiology and Epidemiology
2. Academy of Military Medical Science

Abstract: The Hep-G_2 cell lines were inoculated for isolating HEV and two strain CPE Viruses (R19, R25)were isolated from experimental infected monkey s acute phase fecal. IEM revealed that the isolated two strain viruses could be aggregated by acute phase sera of ET-NANBH patients from Xinjiang and USSR respectively and there were obvious antibodies and full, spheric 1 27-34nm virus-like empty particles (VLPS). The two strain viruses could be neutralized by acute phase sera of HE patients. The sera of patients from...