Studies on PCR for Safety-detection of Rabies Virus in Samples

JIN Yu-Huai; JIN Shi-Xiang; WANG Yong-Xiang; WANG Yu-Kun; GU Bao-Liang

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February 1993

JIN Yu-Huai, JIN Shi-Xiang, WANG Yong-Xiang, WANG Yu-Kun and GU Bao-Liang. Studies on PCR for Safety-detection of Rabies Virus in Samples[J]. Virologica Sinica, 1993, 8(1).

Citation: JIN Yu-Huai, JIN Shi-Xiang, WANG Yong-Xiang, WANG Yu-Kun, GU Bao-Liang. Studies on PCR for Safety-detection of Rabies Virus in Samples .VIROLOGICA SINICA, 1993, 8(1) : 114.

多聚酶链反应检测狂犬病毒的研究

1.
河北医学院微生物学教研室
2.
河北医学院微生物学教研室石家庄
3.
石家庄

摘要

应用一对狂犬病毒特异性引物,经逆转录和循环的扩增,从狂犬病毒感染的细胞及加热灭活的这种样本中,都扩增出了与预期相符的带,表明结合热灭活技术可用作狂犬病毒的安全,敏感,特异,快速检测手段。
- 多聚酶链反应
- , 狂犬病毒
- , 热灭活
- , 检测

Studies on PCR for Safety-detection of Rabies Virus in Samples

1.
Department of Microbiology
2.
Hebei Medical College
3.
Shijiazhuang

Abstract

Polymerase Chain Reaction (PCR) combined with Heat-inactivation is adopted to detect of rabies virus. Using a pair of primers specific for rabies virus, reverse transcription and PCR were performed. The amplified products were visualized after electrophoresis on 2% agarose gel, stained with ethidium bromide and exposed to ultroviolet light. It is shown that:(1) A DNA fragment corresponding in size to the distance between the two primers (329 bp) could be amplified from the nucleic acid extracted from the ra...
- PCR
- , Rabies virus
- , Heat-inactivation
- , Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on PCR for Safety-detection of Rabies Virus in Samples

1. Department of Microbiology
2. Hebei Medical College
3. Shijiazhuang

Abstract: Polymerase Chain Reaction (PCR) combined with Heat-inactivation is adopted to detect of rabies virus. Using a pair of primers specific for rabies virus, reverse transcription and PCR were performed. The amplified products were visualized after electrophoresis on 2% agarose gel, stained with ethidium bromide and exposed to ultroviolet light. It is shown that:(1) A DNA fragment corresponding in size to the distance between the two primers (329 bp) could be amplified from the nucleic acid extracted from the ra...