A Study on the NPV of Oxyplax ochracea and Analysis of Its DNA Map with Restriction Endonucleases

SHANG Xian-Chun; LIANG Shi-Ping; SUN Fu-Lin

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April 1993

SHANG Xian-Chun, LIANG Shi-Ping and SUN Fu-Lin. A Study on the NPV of Oxyplax ochracea and Analysis of Its DNA Map with Restriction Endonucleases[J]. Virologica Sinica, 1993, 8(2).

Citation: SHANG Xian-Chun, LIANG Shi-Ping, SUN Fu-Lin. A Study on the NPV of Oxyplax ochracea and Analysis of Its DNA Map with Restriction Endonucleases .VIROLOGICA SINICA, 1993, 8(2) : 158.

斜纹刺蛾核型多角体病毒及其核酸的限制性内切酶酶解分析

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

本文描述了从自然罹死的斜纹刺蛾(Oxyplax orhracea(moore))幼虫中分离出一种核型多角体病毒。用快速简便方法提取核酸,经限制性内切酶EcoRⅠ、HindⅢ酶解,获得该病毒核酸的酶解带谱。以λDNA的EcoRⅠ酸解片段在凝胶中的迁移率与相应DNA片段分子量的对数值做标准曲线求得其平均分子量为102.09×10~6道尔顿,即为147.77kb。
- 斜纹刺蛾核型多角体
- , 形态学
- , 限制性内切酶

A Study on the NPV of Oxyplax ochracea and Analysis of Its DNA Map with Restriction Endonucleases

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

The morphology of Oxyplax ochracea (Moore) Nuclear Polyhedrosis Virus (OoNPV) isolated from naturally infected larvae is described in this paper. The endonuclease digestion map of OoNPV-DNA is obtained by restriction endonucleases EcoRI and HindⅢ digesting, The average molecular weight of OoNPV is 102.0919~6d, i.e. 147.77kb.
- Oxyplaz ochracea Nuclear Polyhedrosis Virus Morphology Restriction endonucleases map

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Study on the NPV of Oxyplax ochracea and Analysis of Its DNA Map with Restriction Endonucleases

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: The morphology of Oxyplax ochracea (Moore) Nuclear Polyhedrosis Virus (OoNPV) isolated from naturally infected larvae is described in this paper. The endonuclease digestion map of OoNPV-DNA is obtained by restriction endonucleases EcoRI and HindⅢ digesting, The average molecular weight of OoNPV is 102.0919~6d, i.e. 147.77kb.