ZHANG Bao-Yan and KE Li-Hua. Studies on in Vivo Translation of Grass Carp Hemorrhage Virus Genome[J]. Virologica Sinica, 1993, 8(2).
Citation:
ZHANG Bao-Yan, KE Li-Hua.
Studies on in Vivo Translation of Grass Carp Hemorrhage Virus Genome .VIROLOGICA SINICA, 1993, 8(2)
: 185.
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摘要
本文采用α-[~(32)p]ATP标记物在鱼肾细胞系(CIK)系统中,对草鱼出血病病毒(Grass carp hemorrhage virus,GCHV)基因组进行了体内转录的研究。通过放线菌素D抑制宿主细胞基因组的转录活动,从感染病毒细胞中分离出病毒的mRNA,分别采用液相杂交和Nortbern blot方法检查病毒mRNA的转录情况。试验结果表明,GCHV含有内源性转录酶,其基因组的转录活动是在病毒感染细胞后4小时开始,由早期基因所转录,8—10小时获得晚期基因的转录产物。这些mRNA的大小、数目大体上与病毒基因组一致。
Studies on in Vivo Translation of Grass Carp Hemorrhage Virus Genome
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Abstract
Studies on grass carp hemorrhage virus (GCHV) genome transcription in vivo have been carried out Viral mRNA labeled with α-~(32)p ATP was extracted from infected cells treated with actinomycin D and detected by means of liquid phase hybridization and Northern blot hybridization. The results showed that the early and late stage mRNA were transcribed at 4hr and 8hr after infection and roughly divided into four groups, among which each one corresponds to the relative viral genome segment.
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References
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Proportional views
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