Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies

ZHANG Dong-Hai

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June 1993

ZHANG Dong-Hai. Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies[J]. Virologica Sinica, 1993, 8(3).

Citation: ZHANG Dong-Hai. Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies .VIROLOGICA SINICA, 1993, 8(3) : 207.

改良抗体捕获ELISA方法检测流行性出血热特异性IgE、IgA、IgG抗体的初步研究

张东海

1.
山东省淄博第二卫生学校淄博
2.
5015

摘要

初步报道建立了一种检测流行性出血热(EHF)特异性IgE、IgA、IgG抗体的改良抗体捕获ELISA方法(EacELISA,AacELISA,GacELISA)。该法以抗人IgE、IgA或IgG单克隆抗体作包被抗体;酶标记物系两株组特异性较强的EHF?McAb(A (35),A (25-1)株);在实验中采用EHF病毒抗原与酶标记物混合后一次加入,而不是分别依次加入的方式,使操作步骤简化,实验时间缩短,又适当提高了试验敏感性。该法具有简便,特异性高,灵敏度较高(检测EHF?IgE,EHF?IgA>1:100;EHF?IgG>1:2560),重复性较好(CV:EHF?IgE 2.51％,EHF?IgA 11.80％,EHF?IgG 10.85％)等优点。检测39份EHF病人血清,急性期病人(3～7病日)血清三种抗体的检出率分别为84.21％(16/19,EHF?IgG),89.47％(17/19,EHF?IgA)和100％(19/19,EHF?IgG);而发病3～6月后患者血清三种抗体检出率分别为10.00％(2/20),45.00％(9/20)和100.00％。在急性期与恢复期病人之间,EHF?IgE和EHF...
- 流行性出血热
- , IgE抗体
- , IgA抗体
- , IgG抗体
- , 抗体捕获酶联试验

Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies

ZHANG Dong-Hai

1.
Zibo Second Health School
2.
Zibo

Abstract

In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP conjugates. In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously. Thirty-nine EHF patient s sera were tested by this ...
- Epidemic hemorrhagic fever （EHF）
- , IgE IgA IgG antibody
- , Improved antibody capture ELISA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies

ZHANG Dong-Hai

1. Zibo Second Health School
2. Zibo

Abstract: In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP conjugates. In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously. Thirty-nine EHF patient s sera were tested by this ...