Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts

CHEN Qi-Nan; ZHENG Guan-Biao; LIN Kong-Xun; FAN Fu-Zhong

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June 1993

CHEN Qi-Nan, ZHENG Guan-Biao, LIN Kong-Xun and FAN Fu-Zhong. Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts[J]. Virologica Sinica, 1993, 8(3): 263-270.

Citation: CHEN Qi-Nan, ZHENG Guan-Biao, LIN Kong-Xun, FAN Fu-Zhong. Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts .VIROLOGICA SINICA, 1993, 8(3) : 263-270.

番木瓜环斑病毒在番木瓜原生质体中复制的初步研究

1.
华南农业大学植保系
2.
华南农业大学植保系广州
3.
0642 深圳动植物检疫局工作
4.
0642

摘要

以1.0％的Macerozyme R10,1.5％的Cellulose Onozuka R10,0.45mol/L的甘露醇和pH5.4的培养基作为分离番木瓜原生质体的最佳条件,得到了高活力和高产量的原生质体。PRV-Ys(Vb)株系和聚鸟氨酸(PLO,WW200000)分别以1g/ml的最终浓度接种番木瓜原生质体(510~6原生质体/ml),成功地建立了PRV番木瓜原生质体体系。对于PRV在番木瓜原生质体中复制的行为动态差异,本实验研究了PRV-Ys和PRV-Vb两株系,三个不同抗性梯度的番木瓜原生质以及不同培养温度之间相互作用,相互影响的关系,从而认为PRV不仅能在寄主品种Carica papaya var,F1和C.papaya var.Tw-5而且还能在非寄主C.stipulata的原生质体中进行增殖;环境温度不仅能影响PRV在番木瓜细胞中的增殖能力,而且还能影响植株细胞本身的生活能力和抗PRV侵染的能力;PRV-Ys和PRV-Vb两株系在C.papaya var.F1原生质体中的增殖潜伏期和增殖曲线没有明显差异。
- 抗性表达
- , 病毒原生质体体系
- , 单细胞侵染模型

Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts

1.
Plant Protection Department
2.
South-China Agricullure University
3.
Guangzhou
4.
0642

Abstract

A concentration of 110~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was successfully carried out by exposing 510~5 cells/ml of the protoplasts to the final mixture of 1 g/ml PRV-Ys (or Vb) strains and 1g/ml poly-L-orintine (MW200000). The preliminary results of the studies on behavior kinecti...
- Resistance expressionn
- , Virus-protoplast system
- , Model of single-cell infection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts

1. Plant Protection Department
2. South-China Agricullure University
3. Guangzhou
4. 0642

Abstract: A concentration of 110~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was successfully carried out by exposing 510~5 cells/ml of the protoplasts to the final mixture of 1 g/ml PRV-Ys (or Vb) strains and 1g/ml poly-L-orintine (MW200000). The preliminary results of the studies on behavior kinecti...