Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene

LI Ping-Ping; CHEN Ai-Min; SHU Jia-Hong

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June 1993

LI Ping-Ping, CHEN Ai-Min and SHU Jia-Hong. Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene[J]. Virologica Sinica, 1993, 8(3): 293-295.

Citation: LI Ping-Ping, CHEN Ai-Min, SHU Jia-Hong. Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene .VIROLOGICA SINICA, 1993, 8(3) : 293-295.

用Lac基因筛选表达狂犬病毒糖蛋白的重组痘苗病毒

1.
卫生部武汉生物制品研究所
2.
卫生部武汉生物制品研究所武汉
3.
0060

摘要

为了研制基因工程狂犬病疫苗,我国于1991年首次报道了在痘苗病毒天坛株中表达狂犬病毒糖蛋白,但报道中重组病毒的选择是先经人骨髓瘤细胞(TK-143)在诱变剂5-溴脱氧尿苷(BrudR)作用下通过标记拯救技术筛选出携带有同源基因的重组病毒,然后再利用重组病毒中携带的Lac基因为选择标记,通过噬斑纯化获得重组病毒,用这种选择方式获得的重组病毒,经过了TK-143细胞和BrudR,因此不宜发展成疫苗,本研究探索不经过TK-143细胞和BrudR,仅利用Lac基因为选择标记,直接在鸡胚细胞上通过噬斑纯化获得重组病毒,现将研究结果报道如下。
- 狂犬病毒糖蛋白基因
- , 重组痘苗病毒

Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene

1.
Wuhan Institute of Biological Products
2.
Wuhan 430060

Abstract

The expression plasmid pWS-4 containing Rabies virus glycoprotein gene was recombinaed with vaccinia viruses in the presence of Lipofectin~(TM) Reagent by homologous recombination technique in chicken embryonic cells. The Lac gene on the recombinant was used as a selected marker for isolation of TK recombinants with blue plaque on cells. Dot blot showed that the rabies virus glycoprotein gene was inserted into vaccinia genome. The recombinant was responsed specifically with McAb of rabies virus ERA strain b...
- Rables virus glycoprotein gene
- , Recombinant vaccinia virus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene

1. Wuhan Institute of Biological Products
2. Wuhan 430060

Abstract: The expression plasmid pWS-4 containing Rabies virus glycoprotein gene was recombinaed with vaccinia viruses in the presence of Lipofectin~(TM) Reagent by homologous recombination technique in chicken embryonic cells. The Lac gene on the recombinant was used as a selected marker for isolation of TK recombinants with blue plaque on cells. Dot blot showed that the rabies virus glycoprotein gene was inserted into vaccinia genome. The recombinant was responsed specifically with McAb of rabies virus ERA strain b...