Studies on the Purification and Biochemical Properties of BaYMV

DIAO Xiao-Li; TUN Jian-Hua; HUANG Chun-Nong; WENG Cheng-Hua; GONG Jie-Xun

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June 1993

DIAO Xiao-Li, TUN Jian-Hua, HUANG Chun-Nong, WENG Cheng-Hua and GONG Jie-Xun. Studies on the Purification and Biochemical Properties of BaYMV[J]. Virologica Sinica, 1993, 8(3): 296-300.

Citation: DIAO Xiao-Li, TUN Jian-Hua, HUANG Chun-Nong, WENG Cheng-Hua, GONG Jie-Xun. Studies on the Purification and Biochemical Properties of BaYMV .VIROLOGICA SINICA, 1993, 8(3) : 296-300.

大麦黄花叶病毒(BaYMV)的提纯和生化性质的研究

1.
中国科学院上海生物化学研究所
2.
杭州大学生物系
3.
中国科学院上海生物化学研究所进修教师
4.
0031
5.
0028

摘要

大麦黄花叶病是由禾谷多粘菌(polymyxa graminis)传布的一类麦类病毒病。是上海市效区及长江中下游及沿海大麦产区的重要病害。大麦黄花叶病毒(BaYMV)在国内外虽已有不少研究,但对国内分离株的生化性质尚未有详细报道。本文报道以上海郊区的BaYMV为研究材料,成功地建立了一套分离提纯的方法,并在此基础上,研究了该病毒的结构蛋白和核酸性质,并和国外的一些研究结果进行了比较,从而为今后应用生物技术防治大麦黄花叶病毒打下了基础。
- 大麦黄花叶病毒
- , 外壳蛋白
- , 核酸基因组

Studies on the Purification and Biochemical Properties of BaYMV

1.
Shanhai Institute of Biochemistry
2.
Academia Sinica
3.
Shanghai
4.
0031Departement of Biology
5.
Hangzhou University

Abstract

The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ty gradient centrifugation. The isolate showed the existence of two peaks in particle-length distribution (300 nm and 600 nm). SDS-PAGE of purified virus preparation demonstrated one major band of 29kd. A minor b...
- Barley Yellow Mosaic Virus
- , Coat protein
- , Viral RNA genome

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on the Purification and Biochemical Properties of BaYMV

1. Shanhai Institute of Biochemistry
2. Academia Sinica
3. Shanghai
4. 0031Departement of Biology
5. Hangzhou University

Abstract: The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ty gradient centrifugation. The isolate showed the existence of two peaks in particle-length distribution (300 nm and 600 nm). SDS-PAGE of purified virus preparation demonstrated one major band of 29kd. A minor b...