cDNA Cloning and Sequencing Full-Length Genome of Hepatitis E XT-179 Virus Strain Isolated in China

MA Hua-Yin; CAO Hua-Xi; NA Tian-Qi-He; Thin Thin Aye; ZHI Fang-Dun-Fu

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August 1993

MA Hua-Yin, CAO Hua-Xi, NA Tian-Qi-He, Thin Thin Aye and ZHI Fang-Dun-Fu. cDNA Cloning and Sequencing Full-Length Genome of Hepatitis E XT-179 Virus Strain Isolated in China[J]. Virologica Sinica, 1993, 8(4): 316-326.

Citation: MA Hua-Yin, CAO Hua-Xi, NA Tian-Qi-He, Thin Thin Aye, ZHI Fang-Dun-Fu. cDNA Cloning and Sequencing Full-Length Genome of Hepatitis E XT-179 Virus Strain Isolated in China .VIROLOGICA SINICA, 1993, 8(4) : 316-326.

戊型肝炎病毒中国XT-179株全基因组cDNA克隆及其核苷酸和氨基酸序列分析

1.
新疆维吾尔自治区卫生防疫站
2.
日本大学医学部第一病理学教室
3.
缅甸联邦仰光总医院
4.
日本大学医学部第一病理学教室乌鲁木齐
5.
乌鲁木齐

摘要

本文报道对中国新疆东部一起戊型肝炎(HE)流行高峰期间,由HE患者粪便中分离到的戊型肝炎病毒(HEV)中国XT-179株进行全基因组cDNA克隆、核苷酸和氨基酸序列测定及分析结果。所阐明的HEV-XT-179株基因组全序列由7194个核苷酸和3端的多聚腺嘌呤核苷酸[poly(A)]尾组成。四种核苷酸的含量腺嘌呤(A)为17.0％,胞嘧啶(C)为31.9％,鸟嘌呤(G)为26.0％,尿嘧啶(U)为25.1％。G+C含量为57.9％。全基因组含有9个限制性内切酶位点,其基因编码区三个开读框架分别编码了1693,660和123个氨基酸的序列,从而构成了HEV基因组结构区和非结构区的三个多聚蛋白。将其基因序列与HEV缅甸ET1.1株比较,二者之间有395个核苷酸和42个氨基酸以及1个限制性内切酶位点不同,其核苷酸及氨基酸的同源性分别为94.5％和98.3％。以上结果提示不同地区、不同时期分离到的HEV流行株可能存在不同的基因型别和抗原结构的差异。
- 戊型肝炎病毒
- , cDNA克隆
- , 核苷酸序列
- , 氨基酸序列

cDNA Cloning and Sequencing Full-Length Genome of Hepatitis E XT-179 Virus Strain Isolated in China

1.
Xinjiang Hygiene and Anti-epidemic Stalion
2.
Wulumuqi 830011

Abstract

A total of 7.2 kb of full-length genome of hepatitis E virus (HEV)XT-179 strain was cloned as cDNAs that isolated from a HE patient s fecal sample during an epidemic outbreak peak of Sept. 1990 in the East Xinjiang of China, and the complete nucleotide sequence has been determined. This strain have a genome length of 7194 nucleotides followed by a poly(A)tail. The nucleotide composition of the HEV genomic RNA is 17.0% A,31.9%C,26.0%G and 25.1%U,conferring an overall G+C content of 57.9%. There are mine res...
- Hepatitis E virus
- , cDNA cloning
- , Nucleotide sequence
- , Amino acid sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

cDNA Cloning and Sequencing Full-Length Genome of Hepatitis E XT-179 Virus Strain Isolated in China

1. Xinjiang Hygiene and Anti-epidemic Stalion
2. Wulumuqi 830011

Abstract: A total of 7.2 kb of full-length genome of hepatitis E virus (HEV)XT-179 strain was cloned as cDNAs that isolated from a HE patient s fecal sample during an epidemic outbreak peak of Sept. 1990 in the East Xinjiang of China, and the complete nucleotide sequence has been determined. This strain have a genome length of 7194 nucleotides followed by a poly(A)tail. The nucleotide composition of the HEV genomic RNA is 17.0% A,31.9%C,26.0%G and 25.1%U,conferring an overall G+C content of 57.9%. There are mine res...