Studies on Inhibition of Antisense Phosphorothioate Oligodeoxynucleotides on Expression of HBsAg and HBeAg in Cell Culture System

SHANG Hua; LIN Zhong-Yuan; ZHANG Hao; ZONG Jian-Chao; NI Ai-Guo; LIU Fu-Sen

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August 1993

SHANG Hua, LIN Zhong-Yuan, ZHANG Hao, ZONG Jian-Chao, NI Ai-Guo and LIU Fu-Sen. Studies on Inhibition of Antisense Phosphorothioate Oligodeoxynucleotides on Expression of HBsAg and HBeAg in Cell Culture System[J]. Virologica Sinica, 1993, 8(4): 327-331.

Citation: SHANG Hua, LIN Zhong-Yuan, ZHANG Hao, ZONG Jian-Chao, NI Ai-Guo, LIU Fu-Sen. Studies on Inhibition of Antisense Phosphorothioate Oligodeoxynucleotides on Expression of HBsAg and HBeAg in Cell Culture System .VIROLOGICA SINICA, 1993, 8(4) : 327-331.

反义寡聚硫代磷酸脱氧核苷抑制体外细胞培养系统中乙肝抗原表达的研究

1.
天津医学院微生物学教研室
2.
南开大学分子生物学研究所
3.
南开大学分子生物学研究所天津 300070上海医科大学博士后

摘要

设计合成了两个分别互补于乙肝病毒2.1kb mRNA起始区(片段A)和增强子区(片段B)的硫代磷酸的DNA片段。在经克隆HBV DNA转染HepG_2细胞建立的HBV短暂表达系统及稳定产生HBV的2215细胞中研究二者对HBsAg及HBeAg表达的抑制作用。结果表明反义寡聚物能不同程度抑制乙肝抗原表达,并与剂量呈一定正相关。在HepG_2细胞HBV短暂表达系统中,6mol/L浓度时,片段A、B对HBsAg的抑制作用分别为60％,56.1％,对HBeAg表达的抑制水平分别为45％、48％。在2215细胞中,18mol/L浓度时,二者对HBsAg及HBeAg的抑制作用只有20～35％左右。同时采用甲基蓝四氮唑(MTT)方法检测了反义寡聚物对HepG_2细胞活性的影响,在高浓度40mol/L时才呈现15％左右的抑制作用,这表明其抑制乙肝抗原的表达是高选择性的。
- 乙肝病毒
- , 反义DNA
- , 细胞培养
- , 基因调控
- , 抗病毒制剂

Studies on Inhibition of Antisense Phosphorothioate Oligodeoxynucleotides on Expression of HBsAg and HBeAg in Cell Culture System

1.
Department of Microbiology
2.
Tianjin Medical College
3.
Tianjin 300070

Abstract

Antisense phosphorothioate oligodeoxynueleotides respectively complementary to initial region of 2.1kb mRNA(oligomer A,14nts)and enhancer I(oligomer B,12nts)of HBV were synthesed by chemi cal synthetic method,and inhibition of these oligomers on expression of HBsAg and HBeAg in cell cul ture system was studied. The oligomer A inhibited more than 50％ of HBsAg at 3mol/L, but the oligomer B did at 6mol/L. On the contrary,the oligomer B showed 35％ inhibition on expression of HBeAg at 3mol/L,but 22% the oligo...
- Hepatitis B virus
- , Antisense DNA
- , Gene regulation
- , Cell culture
- , Antiviral agents

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Inhibition of Antisense Phosphorothioate Oligodeoxynucleotides on Expression of HBsAg and HBeAg in Cell Culture System

1. Department of Microbiology
2. Tianjin Medical College
3. Tianjin 300070

Abstract: Antisense phosphorothioate oligodeoxynueleotides respectively complementary to initial region of 2.1kb mRNA(oligomer A,14nts)and enhancer I(oligomer B,12nts)of HBV were synthesed by chemi cal synthetic method,and inhibition of these oligomers on expression of HBsAg and HBeAg in cell cul ture system was studied. The oligomer A inhibited more than 50％ of HBsAg at 3mol/L, but the oligomer B did at 6mol/L. On the contrary,the oligomer B showed 35％ inhibition on expression of HBeAg at 3mol/L,but 22% the oligo...