In Vitro and in Vivo Heptatitis B Virus (HBV) Inhibiting Bone Marrow Mononuclear Cells Producing Interleukin-1 and Interleukin-2

WANG Jian-Hua; XIONG Kai-Jun; HUANG Hua-Fang

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August 1993

WANG Jian-Hua, XIONG Kai-Jun and HUANG Hua-Fang. In Vitro and in Vivo Heptatitis B Virus (HBV) Inhibiting Bone Marrow Mononuclear Cells Producing Interleukin-1 and Interleukin-2[J]. Virologica Sinica, 1993, 8(4): 332-337.

Citation: WANG Jian-Hua, XIONG Kai-Jun, HUANG Hua-Fang. In Vitro and in Vivo Heptatitis B Virus (HBV) Inhibiting Bone Marrow Mononuclear Cells Producing Interleukin-1 and Interleukin-2 .VIROLOGICA SINICA, 1993, 8(4) : 332-337.

乙型肝炎病毒(HBV)体内外对单个核细胞白细胞介素-1和白细胞介素-2诱生活性影响的研究

1.
同济医科大学协和医院传染科
2.
同济医科大学协和医院传染科武汉 430022广东佛山石湾医院传染科

摘要

本研究用PAP法、胸腺细胞增殖法、脾细胞增殖法,分别检测16例体外HBV感染的骨髓单个核细胞与16例慢性乙型肝炎患者体内感染的骨髓单个核细胞(MNCs)中的HBcAg和白细胞介素-1(IL-1)、白细胞介素-2(IL-2)的诱生活性(以△cpm值表示)。结果显示,体外HBV感染组与体内HBV感染组骨髓MNCs中HBcAg检出率分别为50％和43.7％。本实验结果表明,HBV在体外感染骨髓MNCs,且与体内自然感染相符,但光镜下未观察到致细胞病变效应(CPE)。体外感染组与体内感染组IL-I和IL-2活性均较对照组明显下降(P0.01)。且细胞中HBcAg检出阳性者较阴性者下降更为明显(P0.01)。IL-1和IL-2诱生活性降低与HBV侵染免疫细胞及其在细胞内复制有密切关系,从而提示,IL-1和IL-2降低可能影响HBV的清除而引起慢性化过程。
- 骨髓单个核细胞
- , 白细胞介素-1
- , 白细胞介素-2
- , 乙型肝炎病毒
- , 复制

In Vitro and in Vivo Heptatitis B Virus (HBV) Inhibiting Bone Marrow Mononuclear Cells Producing Interleukin-1 and Interleukin-2

1.
Dept of Infecious Diseases
2.
Union Hospilal

Abstract

The levels of interleukin-1 and interleukin-2 production in 16 healthy blood donors bone mar row mononuclear cells infected by HBV in vitro and on 16 cases with chronic hepatitis B were simulta neously assayed. The evidence of virus growth is based on the detecion of HBcAg in bone marrow mononuclear cells by immunoperoxidase assay. It was found that HBcAg is positive in 50% (in vitro) and 43. 7％(in vivo) respectively (P0.05). The levels of interleukin-1 and interleukin-2 produetion are markedly decreas...
- Interleukin-1
- , Interleukin-2
- , Bone marrow mononuclear cell
- , Hepatitis B virus
- , Replication

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

In Vitro and in Vivo Heptatitis B Virus (HBV) Inhibiting Bone Marrow Mononuclear Cells Producing Interleukin-1 and Interleukin-2

1. Dept of Infecious Diseases
2. Union Hospilal

Abstract: The levels of interleukin-1 and interleukin-2 production in 16 healthy blood donors bone mar row mononuclear cells infected by HBV in vitro and on 16 cases with chronic hepatitis B were simulta neously assayed. The evidence of virus growth is based on the detecion of HBcAg in bone marrow mononuclear cells by immunoperoxidase assay. It was found that HBcAg is positive in 50% (in vitro) and 43. 7％(in vivo) respectively (P0.05). The levels of interleukin-1 and interleukin-2 produetion are markedly decreas...