The TK Gene of Raccoon Pox Virus is not Sensitive to 5-BUdR

DIAO Wei-Guo; Joseph J.Esposito

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August 1993

DIAO Wei-Guo and Joseph J.Esposito. The TK Gene of Raccoon Pox Virus is not Sensitive to 5-BUdR[J]. Virologica Sinica, 1993, 8(4): 338-340.

Citation: DIAO Wei-Guo, Joseph J.Esposito. The TK Gene of Raccoon Pox Virus is not Sensitive to 5-BUdR .VIROLOGICA SINICA, 1993, 8(4) : 338-340.

浣熊痘病毒TK基因对5-BUdR药物不敏感性的研究

1.
卫生部武汉生物制品研究所
2.
美国疾病控制研究中心武汉

摘要

本文在Rat-2(TK~-)细胞上,对浣熊痘病毒TK基因作为病毒筛选标记的作用进行了研究。发现浣熊痘病毒虽然具有TK基因的序列和功能,但对5-溴脱氧尿嘧啶(5-BUdR)药物的选择压力并不敏感。即使采用HAT培养基挑选的单斑病毒对5-BUdR也具有抵抗力。
- 浣熊痘病毒
- , TK基因
- , 5-BUdR

The TK Gene of Raccoon Pox Virus is not Sensitive to 5-BUdR

1.
1~Wuhan Institute of Biological Products
2.
Wuhan

Abstract

In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. Southern blot showes that RCN has TK gene sequence though it hybridizes week ly with vaccinia virus TK gene. But RCN is resistant to 5-BUdR even after it was plaque purified in HAT media.
- Raccon poxvirus
- , TK gene
- , 5-BUdR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The TK Gene of Raccoon Pox Virus is not Sensitive to 5-BUdR

1. 1~Wuhan Institute of Biological Products
2. Wuhan

Abstract: In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. Southern blot showes that RCN has TK gene sequence though it hybridizes week ly with vaccinia virus TK gene. But RCN is resistant to 5-BUdR even after it was plaque purified in HAT media.