The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli

TAO Hua-Jian; XU Jia-Lin; LIU Yi

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1993

TAO Hua-Jian, XU Jia-Lin and LIU Yi. The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli[J]. Virologica Sinica, 1993, 8(4): 385-389.

Citation: TAO Hua-Jian, XU Jia-Lin, LIU Yi. The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli .VIROLOGICA SINICA, 1993, 8(4) : 385-389.

甜菜坏死黄脉病毒外壳蛋白基因在大肠杆菌中的高效表达

1.
北京农业大学国家农业生物技术重点实验室
2.
北京农业大学国家农业生物技术重点实验室北京

摘要

将甜菜坏死黄脉病毒(BNYVV)内蒙古分离物的外壳蛋白基因亚克隆到pJW2上构建成在大肠杆菌中表达的载体。SDS-PAGE及Westexn blotting检测的结果表明,该表达载体在大肠杆菌DH5 中经温度诱导后特异地表达21kD的甜菜坏死黄脉病毒外壳蛋白。经光密度扫描估测,其表达量占大肠杆菌总蛋白的19.5％
- 甜菜坏死黄脉病毒
- , 外壳蛋白基因
- , 表达

The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli

1.
National Laboratories for Agrobiotechnology
2.
Beijing Agricullural University

Abstract

The temperature-inducible expression vector containing the beet necrotic yellow vein virus(BNYVV)isolate NM coat protein gene was constructed and transferred into E. coli DH5 . High-level expression of the specific protein was achieved by temperature induction. The results of SDSPAGE and Western blotting show that the expression product which accumulates 19.5％ of the total cellular proteins estimated by Shimadzu CS-910 scanning is 21kd BNYVV coat protein.
- BNYW
- , Coat protein gene
- , Expression

References
Proportional views

PDF

Article Metrics

Article views(3591) PDF downloads(744) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The High-level Expression of Beet Necrotic Yellow Vein Virus Coat Protein Gene in Escherichia coli

1. National Laboratories for Agrobiotechnology
2. Beijing Agricullural University

Abstract: The temperature-inducible expression vector containing the beet necrotic yellow vein virus(BNYVV)isolate NM coat protein gene was constructed and transferred into E. coli DH5 . High-level expression of the specific protein was achieved by temperature induction. The results of SDSPAGE and Western blotting show that the expression product which accumulates 19.5％ of the total cellular proteins estimated by Shimadzu CS-910 scanning is 21kd BNYVV coat protein.