Detection of I-IPV- 18 DNA with PCR

JIANG Gong; KE Li-Hua

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Volume 09 Issue 1

February 1994

JIANG Gong and KE Li-Hua. Detection of I-IPV- 18 DNA with PCR[J]. Virologica Sinica, 1994, 9(1).

Citation: JIANG Gong, KE Li-Hua. Detection of I-IPV- 18 DNA with PCR .VIROLOGICA SINICA, 1994, 9(1) : 7.

PCR检测HPV－18DNA

江红 ,
柯丽华

1.
中国科学院武汉病毒研究所

摘要

采用聚合酶链式反应（ＰＣＲ）技术，对ＨＰＶ－１８序列中引物ＨＰ＿１、ＨＰ＿２之间的片段（Ｆ）进行扩增，通过两组阴、阳性对照实验证明扩增片段的特异性。用不同Ｍｇ浓度的缓冲系统进行ＰＣＲ反应发现，缓冲系统中Ｍｇ浓度高低是影响ＨＰＶ－１８／ＨＰ＿１、ＨＰ＿２特异扩增的重要因素，高浓度Ｍｇ导致扩增特异性降低。对１７例宫颈癌组织ＤＮＡ进行ＰＣＲ检测，有９例检出Ｆ片段，其检出率是５３％，为ＨＰＶ－１８与宫颈癌的相关性提供证据。
- 人乳头瘤病毒18型（HPV－18）聚合酶链式反应（PCR）宫颈癌特异性检测

Detection of I-IPV- 18 DNA with PCR

JIANG Gong ,
KE Li-Hua

1.
Wuhan Institute of Virology,Chinese Academy of Science

Abstract

The DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma.
- HPV-18 PCR Cervical carcinoma Specificity Detection

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of I-IPV- 18 DNA with PCR

JIANG Gong ,
KE Li-Hua

1. Wuhan Institute of Virology,Chinese Academy of Science

Abstract: The DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma.