The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils

XU Xu-Shi; ZHANG Chao-Yang; XIE Tian-En

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February 1994

XU Xu-Shi, ZHANG Chao-Yang and XIE Tian-En. The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils[J]. Virologica Sinica, 1994, 9(1).

Citation: XU Xu-Shi, ZHANG Chao-Yang, XIE Tian-En. The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils .VIROLOGICA SINICA, 1994, 9(1) : 37.

BsNPV感染油桐尺蠖及Bs484细胞的酯酶分析

1.
江西大学生物学系江西大学中心实验室中国科学院武汉病毒研究所

摘要

本文使用聚丙烯酰胺凝胶电泳及薄层扫描技术测定了油桐尺蠖Ｂｕｚｕｒａｓｕｐｐｒｅｓｓａｒｉａ和Ｂｓ４８４细胞感染核型多角体病毒后的酯酶（ＥＳＴ）含量及类型的变化。结果表明，油桐尺蠖中肠ＦＳＴ的含量和类型在病毒感染８小时后就有明显的改变，随着感染时间延长，这二项指标都有较大的变化。而Ｂｓ４８４细胞的ＥＳＴ含量变化开始于病毒感染后３小时，细胞ＥＳＴ的类型则无改变。
- BsNPV 感染酯酶

The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils

1.
Biology Department of Jiangxi University,Nanchang 330047

Abstract

By the polyacrylamide gel electrophoresis(PAGE)and thin-layer scanning,the esterases of thefourth instar larvae of the Buzura suppressaria and Bs484 cells were investigated after infection with nuclear pelyhedrosis viruses,The amount of the zymograms for mid-gut esterases in diseased larvae wasfound to be less than that in the healthy ones during the course of the disease,The results showed thatthe changes in type and content of mid-gut esterases were investigated at 8 hours postinfection,and thecharlges became obvious as infective time went on,The changes in content of esterases for Bs484 cellswere determined at 3 hours postinfection with BsNPV,but the type of esterases had no changes.
- BsNPV Infection Esterase

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils

1. Biology Department of Jiangxi University,Nanchang 330047

Abstract: By the polyacrylamide gel electrophoresis(PAGE)and thin-layer scanning,the esterases of thefourth instar larvae of the Buzura suppressaria and Bs484 cells were investigated after infection with nuclear pelyhedrosis viruses,The amount of the zymograms for mid-gut esterases in diseased larvae wasfound to be less than that in the healthy ones during the course of the disease,The results showed thatthe changes in type and content of mid-gut esterases were investigated at 8 hours postinfection,and thecharlges became obvious as infective time went on,The changes in content of esterases for Bs484 cellswere determined at 3 hours postinfection with BsNPV,but the type of esterases had no changes.