GU Hong-Cang, YAN Dun-Yu, LIU Huan-Ting, QIU Bing-Sheng, WANG Jin-Fang and TIAN Bo. Detection of grapevine fanleaf virus by dot-blot hybridization with biotin-labelled GFV-cDNA probe[J]. Virologica Sinica, 1994, 9(1).
Citation: GU Hong-Cang, YAN Dun-Yu, LIU Huan-Ting, QIU Bing-Sheng, WANG Jin-Fang, TIAN Bo. Detection of grapevine fanleaf virus by dot-blot hybridization with biotin-labelled GFV-cDNA probe .VIROLOGICA SINICA, 1994, 9(1) : 48.

生物素标记GFV-cDNA探针的制备及在检测葡萄扇叶病毒上的应用

  • 以整合到质粒中的GFV-cDNA为模板经PCR合成了生物素标记的GFV单、双链探针。用合成的探针对提纯的cFV-RNA_2、感染CFV的昆诺藜叶及18株而萄进行DNA-RNA杂交检测表明:检测提纯病毒RNA_2的灵敏度为1.5pg/斑点,感染GFV的昆诺藜提取液最高稀释度可达40960倍;11株显示典型扇叶症状的样品杂交结果均为阳性,且汁液稀释400~800倍仍能测出,7株不显示典型扇叶症状的葡萄中3株受到GFV的侵染。单、双链探针最适使用浓度分别为1/200及1/100。

Detection of grapevine fanleaf virus by dot-blot hybridization with biotin-labelled GFV-cDNA probe

  • The 5′and 3'-primers of 21 bp of GFV coat protein gene were synthesized according to the GFV-RNA sequence,The cDNA of GFV ccat protein gene was syntheeized with reverse transcriptase using3′-primer and GFV-RNA extracted from the Durified virus as template,Then the ds-cDNA was amplified by PCR technique employing GFV-RNA_2:ss-cDNA as templates and 5',3′-primers and ligated-with pBluesclpt ks.The ds-cDNA were transformed into Ecoli XLI-Blue cells,g the cDNA extracted from the recombinantclones of E. coli cells.The biotin-labelled ss,ds-probes have been used for the detection of purified GFV-RNA,GFV-infeted C,quivoa and 18 grapevine leaves by nucleic acid dot-blot hybridization,The minimal amount ofpurified GFV-RNA_2 which gave the visible signal was 1.5pg/test dot and the maximum dilution ofGFV-infected C,quinoa extract which gave visible signal was 1:40960;11 samples with typical symptoms were,petive and some of them could give the signal when they were diluted 1:400 ̄1:800.3samples out of 7 symptomeless leaves

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    Detection of grapevine fanleaf virus by dot-blot hybridization with biotin-labelled GFV-cDNA probe

    • 1. Shandong Agricultural University

    Abstract: The 5′and 3'-primers of 21 bp of GFV coat protein gene were synthesized according to the GFV-RNA sequence,The cDNA of GFV ccat protein gene was syntheeized with reverse transcriptase using3′-primer and GFV-RNA extracted from the Durified virus as template,Then the ds-cDNA was amplified by PCR technique employing GFV-RNA_2:ss-cDNA as templates and 5',3′-primers and ligated-with pBluesclpt ks.The ds-cDNA were transformed into Ecoli XLI-Blue cells,g the cDNA extracted from the recombinantclones of E. coli cells.The biotin-labelled ss,ds-probes have been used for the detection of purified GFV-RNA,GFV-infeted C,quivoa and 18 grapevine leaves by nucleic acid dot-blot hybridization,The minimal amount ofpurified GFV-RNA_2 which gave the visible signal was 1.5pg/test dot and the maximum dilution ofGFV-infected C,quinoa extract which gave visible signal was 1:40960;11 samples with typical symptoms were,petive and some of them could give the signal when they were diluted 1:400 ̄1:800.3samples out of 7 symptomeless leaves

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