CHEN Xiao-Xu, HOU Chu-Xia and LI Re-Lun. Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection[J]. Virologica Sinica, 1994, 9(2): 95-99.
Citation: CHEN Xiao-Xu, HOU Chu-Xia, LI Re-Lun. Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection .VIROLOGICA SINICA, 1994, 9(2) : 95-99.

生物素标记HBV RNA探针的制备及应用

  • 本文首次采用SP(65)特殊质粒与人类的乙型肝类病毒DNA(HBVDNA)重组,制备了Bio-HBVRNA探针,能特异地与HBVDNA杂交。将该探针与缺口转移(NicHranalatiOn)方法标记的m小HBVDNA探针进行了比较。结果显示出Bio-HBVRNA探针比Bio-HBVDNA探针的敏感性提高10倍。并分别应用两种探针同时检测70例己肝病人血清中HBVDNA,阳性率各为31.42%、28.57%(P>0.25).对Bio-HBVRNA探针应用于临床检测乙肝病毒DNA的效能也进行了初步探讨。

Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection

  • epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P0.25).The clinical application of HBV RNA probes was discusseil preluninarily.

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    Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection

    • 1. Department of Microbiology,Military Animal Medicure Cottege of Shen Yanq Comunend,Da Lian 116013

    Abstract: epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P0.25).The clinical application of HBV RNA probes was discusseil preluninarily.

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