WANG Jia-Wang, HUANG Yong-Xiu, TAN Ye-Beng, LI Shou-Dong and JI Xi-Feng. PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus[J]. Virologica Sinica, 1994, 9(2): 130-137.
Citation: WANG Jia-Wang, HUANG Yong-Xiu, TAN Ye-Beng, LI Shou-Dong, JI Xi-Feng. PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus .VIROLOGICA SINICA, 1994, 9(2) : 130-137.

昆虫杆状病毒衣壳主蛋白基因的PCR扩增克隆和定位

  • 用PCR技术成功地扩增了苜蓿丫纹夜蛾核型多角体病毒(AcNPV)的衣壳主蛋白基因(vp39基因),并克隆了该基因,利用纯的vp39基因探针,在低严谨杂交条件下,已将粘虫核型多角体病毒(LsNPV)的vp39基固定位在PstI-F,BamHI-C,EcoRI-C,XhoI-D,I,EcoRV-H,X等片段上。PCR反应时,在扩增出预期的包括完整vp39基因的1406bp片段的同时也扩增出一条Ca.400bp的片段,本文讨论了PCR的特异性扩增和非特异性扩增。

PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus

  • he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.

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    PCR Amplification CloningProtein Gene and Location of Major Capsid Of Baculovirus

    • 1. Institute of Hology ,Wuhan University ,Wuhan 430072

    Abstract: he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been leeated on PstI-F.BamHI-C,EcoRI-C,E,XhoI-D,I,EcoRV-H,X fragnients by Southern blot.Besides amplification of the predicted 1406bp fragment including intact major capsid proteingene of AcNPV using XhoI and HindⅢ-digested AcNPV DNA as a teniplate.a ca.400 bp fragmenthas been amplified.In this paper we disscused the PCR amplification of ca.400 bp fragment of bothspecificity and nonspocificity.

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