Isolation and Detection of Spherical Viruses from Penaells orientalis K

CHEN Dai-Hua; ZHANG Jian-Gong; DAN Zheng-Li; GAO Wei; GAO Hua-Xin; TUN Zhi-An

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April 1994

CHEN Dai-Hua, ZHANG Jian-Gong, DAN Zheng-Li, GAO Wei, GAO Hua-Xin and TUN Zhi-An. Isolation and Detection of Spherical Viruses from Penaells orientalis K[J]. Virologica Sinica, 1994, 9(2): 170-173.

Citation: CHEN Dai-Hua, ZHANG Jian-Gong, DAN Zheng-Li, GAO Wei, GAO Hua-Xin, TUN Zhi-An. Isolation and Detection of Spherical Viruses from Penaells orientalis K .VIROLOGICA SINICA, 1994, 9(2) : 170-173.

中国对虾中一种球状病毒的分离提纯与检测

1.
中国科学院武汉病毒研究所，武汉43007I

摘要

从人工养殖生长比较缓慢的对虾与病虾的肝胰腺和消化道中，分离出一种大小为８０ｎｍ的球状病毒。同时采用超薄切片技术可在对虾中肠细胞中发现大量的球状病毒，这些病毒聚集成片，在同一细胞内的细胞器，如线粒体等均发生病变。此外，将分离提取的球状病毒进行感染试验，感染死亡率为６０％。并且从感染致死的对虾消化腺及肠道中，同样可以观察到大小相同、形态一致的球状病毒颗粒。
- 中国对虾
- , 肝胰腺
- , 球状病毒
- , 分离提取
- , 感染试验

Isolation and Detection of Spherical Viruses from Penaells orientalis K

1.
Wuhan Institute of virology ,Academia

Abstract

uring the cultivition of Penaeus orientalis K,outbreak of death or slowing down of growth couldoccurred sometimes.tuge amount of dispersive spherical viruses with diameter around 80nm were observed in the midgut cells of those sick shrimps under electron microscope.Hoat cell organelles,such asmitochondria,became over swollen,and even broken.The virus was isolated from the hepotopancreasand digestive tube of the sick shrimp and the viriolls were icosahedron with diameter of 80nm on the av-erage.From infection experiment the corrected mortality was as high as 60%.The salne morphological spherical virus with the same size was also isolated from infected died shrimps digeetive glands andtubes.
- Penaeus orientalis K
- , Hepatopancreas
- , Spherical viruses
- , Isolation and extraction
- , Infection experiment

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation and Detection of Spherical Viruses from Penaells orientalis K

1. Wuhan Institute of virology ,Academia

Abstract: uring the cultivition of Penaeus orientalis K,outbreak of death or slowing down of growth couldoccurred sometimes.tuge amount of dispersive spherical viruses with diameter around 80nm were observed in the midgut cells of those sick shrimps under electron microscope.Hoat cell organelles,such asmitochondria,became over swollen,and even broken.The virus was isolated from the hepotopancreasand digestive tube of the sick shrimp and the viriolls were icosahedron with diameter of 80nm on the av-erage.From infection experiment the corrected mortality was as high as 60%.The salne morphological spherical virus with the same size was also isolated from infected died shrimps digeetive glands andtubes.