Partial genome sequence analysis of hepatitis E .virus isolated by cell culture

CHEN Wan-Rong; TIAN Xin; BAO Zuo-Xi; LI Jing-Yun; DENG Da-Rong

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June 1994

CHEN Wan-Rong, TIAN Xin, BAO Zuo-Xi, LI Jing-Yun and DENG Da-Rong. Partial genome sequence analysis of hepatitis E .virus isolated by cell culture[J]. Virologica Sinica, 1994, 9(3).

Citation: CHEN Wan-Rong, TIAN Xin, BAO Zuo-Xi, LI Jing-Yun, DENG Da-Rong. Partial genome sequence analysis of hepatitis E .virus isolated by cell culture .VIROLOGICA SINICA, 1994, 9(3) : 213.

戊型肝炎病毒细胞分离株部分核苷酸序列分析

1.
北京军事医学科学院微生物流行病研究所新疆军区军事医学研究所

摘要

用抗原捕获／多聚酶链反应（ＡＣ／ＰＣＲ）对戊型肝炎病毒（ＨＥＶ）细胞分离株ＭＪ９０和Ｒ２５基因组的部分核苷酸序列进行扩增，获得了与ＨＥＶ缅甸株ＥＴ１·１相同的ｃＤＮＡ扩增带。该ｃＤＮＡ扩增带纯化后用双脱氧核苷酸ＤＮＡ链末端终止法测序，ＣＪ９０、Ｒ２５株的核苷酸和氨基酸序列与ＥＴ１·１克隆的同源性分别为９９．６％、１００％和９９％、９９％，从而证明ＭＪ９０和Ｒ２５毒株为ＨＥＶ．
- 细胞培养戊型肝炎病毒（HEV）抗原捕获／多聚酶链反应（AC／PCR）序列测定

Partial genome sequence analysis of hepatitis E .virus isolated by cell culture

Abstract

Genome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid homology between XJ90 R25 and ET1·1 clone were 99.6%,100%and99%,99%,respectively. This result showed that both XJ90 and R25 are hepatitis E virus.
- Cell culture Hepatitis E virus Antigen capture/polymeras chain reaction Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Partial genome sequence analysis of hepatitis E .virus isolated by cell culture

Abstract: Genome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid homology between XJ90 R25 and ET1·1 clone were 99.6%,100%and99%,99%,respectively. This result showed that both XJ90 and R25 are hepatitis E virus.