Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene

CHEN Dong; XIE Tian-En; JIN Feng

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June 1994

CHEN Dong, XIE Tian-En and JIN Feng. Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene[J]. Virologica Sinica, 1994, 9(3).

Citation: CHEN Dong, XIE Tian-En, JIN Feng. Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene .VIROLOGICA SINICA, 1994, 9(3) : 217.

黄地老虎颗粒体病毒DNA片段的克隆与颗粒体蛋白基因的定位

1.
中国科学院武汉病毒研究所中国科技大学生物系

摘要

用ｐＵＣ１９质粒作载体，克隆了黄地老虎颗粒体病毒（Ａｇｒｏｌｉｓｓｅｇｅｔｕｍｇｒａｎｕｌｏｓｉｓｖｉｒｕｓ，简称ＡｓＧＶ）ＤＮＡＰｓｔＩ－Ｄ．Ｅ．Ｆ．Ｇ．Ｈ．Ｊ．Ｋ．等７个片段。以［￣（３２）Ｐ］－ｄＣＴＰ标记的油桐尺蠖核型多角体病毒（Ｂｕｚｕｒａｓｕｐｐｒｅｓｓａｒｉａｎｕｃｌｃａｒｐｏｌｙｈｅｄｒｏｓｉｓｖｉｒｕｓ简称ＢｓＮＰＶ）多角体蛋白基因为探针，在３７℃条件下对ＡｓＧＶ）颗粒体蛋白基因进行了定位，将其分别定位于ＢｓｌⅡ－Ｓ或ＴＰｓＴＩ－Ａ或Ｂ和ＥｃｉＲＩ－Ａ片段上。
- 黄地老虎颗粒体病毒颗粒体蛋白基因 Southern杂交 DNA克隆

Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene

1.
Wuhan Institute of Virology,CAS,Wuhan 430071

Abstract

Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.
- Agrotis segetum granulosis virus Granulin sene Southern hybridization DNA Cloning

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene

1. Wuhan Institute of Virology,CAS,Wuhan 430071

Abstract: Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.