WANG Zhen-Dong, FANG De-Chun, LIU Yu-Le and TIAN Bo. New virus of eggplant mosaic disease in Shenyang[J]. Virologica Sinica, 1994, 9(3).
Citation: WANG Zhen-Dong, FANG De-Chun, LIU Yu-Le, TIAN Bo. New virus of eggplant mosaic disease in Shenyang .VIROLOGICA SINICA, 1994, 9(3) : 226.

茄子花叶病新毒原

  • 1991年从沈阳地区茄子花叶病叶片中分离到了SY-Is分离物。经汁液摩擦接种7个科16种植物,这一分离物可浸染4个科8种植物,但不侵染黄瓜、两葫芦和蚕豆,也不能经桃蚜和萝卜蚜传播。该分离物体外抗性的致死温度(TIP)为90-95℃;稀释限点(DEP)为10 ̄(-6)-10 ̄(-7);体外保毒期(L)为一个月以上。光学显微镜下可见晶状胞质内含体。叶滴法电镜观察可见大小为±300×18nm的杆状病毒粒子。该病病叶汁液与烟草花叶病毒(TMV)普通株抗血清呈明显阳性反应。用TMV的一对引物进行多聚酶链反应(PCR),可扩增出一特异性核酸片段。根据上述实验结果,我们认为引起沈阳地区茄子花叶病的毒原种类为TMV,是茄子花叶病的新毒原。

New virus of eggplant mosaic disease in Shenyang

  • n identification of a mosaic virtis disease of eggplant which occurs in Shenyang was carried outduring 1991 and 1992.The isolate can be transmitted to some plants by mechanical inoculation,butcannot be transniitted by peach aphid(Myzus pericicae Sulzer,Phopalosiphum pseudobrassicae).Host ranse tbets were made on 16 species,but cannot infect Cucumis sativus,Cucurbita pepo and Vicai faba.In eggplant sap,their proporties were thermal inactivation point (TIP)between 90~95℃,dilution and point(DEP)between 10-6~10-7,longevity of leaf extract at room temperature (>25℃ )between one-several montlis.As electron microscopic observation by dip method reveals the particle of the virus as rod-shapedmeasuring±300×18nm,cystalline inclusions were found in cytoplasma of leaf tissue of infectedpeanut by microscope.The isolate could react with the antiserum of TMV by agar gel double diffusion test.Especially,spesific nucleic acid frasment(±200bp)was amplified by the polymerase chain reaction(PCR).According to these characteristic

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    New virus of eggplant mosaic disease in Shenyang

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    Abstract: n identification of a mosaic virtis disease of eggplant which occurs in Shenyang was carried outduring 1991 and 1992.The isolate can be transmitted to some plants by mechanical inoculation,butcannot be transniitted by peach aphid(Myzus pericicae Sulzer,Phopalosiphum pseudobrassicae).Host ranse tbets were made on 16 species,but cannot infect Cucumis sativus,Cucurbita pepo and Vicai faba.In eggplant sap,their proporties were thermal inactivation point (TIP)between 90~95℃,dilution and point(DEP)between 10-6~10-7,longevity of leaf extract at room temperature (>25℃ )between one-several montlis.As electron microscopic observation by dip method reveals the particle of the virus as rod-shapedmeasuring±300×18nm,cystalline inclusions were found in cytoplasma of leaf tissue of infectedpeanut by microscope.The isolate could react with the antiserum of TMV by agar gel double diffusion test.Especially,spesific nucleic acid frasment(±200bp)was amplified by the polymerase chain reaction(PCR).According to these characteristic

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