Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA

FANG Qin; TUN Yun-Tao; CA Yi-Quan

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June 1994

FANG Qin, TUN Yun-Tao and CA Yi-Quan. Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA[J]. Virologica Sinica, 1994, 9(3).

Citation: FANG Qin, TUN Yun-Tao, CA Yi-Quan. Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA .VIROLOGICA SINICA, 1994, 9(3) : 261.

HBV-DNA的·PCR二步法扩增及其快速检测

1.
中国科学院武汉病毒研究所

摘要

ＨＢＶ－ＤＮＡ的ＰＣＲ二步法扩增及其快速检测方勤，吴云涛，蔡宜权（中国科学院武汉病毒研究所，武汉４３００７１）关键词ＨＢＶ－ＤＮＡ，二步温控ＰＣＲ，Ｓｏｕｔｈｅｒｎ杂交，生物素寡聚核苷酸杂交乙型肝炎是危害人类健康的主要疾病之一，其病原常用的检测方法多...
- HBV－DNA 二步温控PCR Southern杂交生物素寡聚核苷酸杂交

Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA

1.
Wuhan Institute of Virology,CAS, Wuhan 430071

Abstract

specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both ￣(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes with PCR preduets,We also explored the sensitivity of the system,which can detect as less asabout 1-2 HBV-DNA molecules.The convenience of the system was demonstrated by the simple twotemperature PCR stepe and fast preducts detection with biotinylated oliunucleotide probe,This systemcan be used for both labortltory and clinical detection of HBV-DNA.
- HBV-DNA Two-step PCR Southern blot Biotinylated oligonucleotide Hybridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA

1. Wuhan Institute of Virology,CAS, Wuhan 430071

Abstract: specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both ￣(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes with PCR preduets,We also explored the sensitivity of the system,which can detect as less asabout 1-2 HBV-DNA molecules.The convenience of the system was demonstrated by the simple twotemperature PCR stepe and fast preducts detection with biotinylated oliunucleotide probe,This systemcan be used for both labortltory and clinical detection of HBV-DNA.