A Study on Some Biochemical Characteristics of Nuclear
Polyhedrosis Virus of Ectropis grisescens warren

CAI Jian-He; HONG Yi-Guo; HUANG Fu-Xin; WANG Xiao-Feng; TIAN Bo

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June 1994

CAI Jian-He, HONG Yi-Guo, HUANG Fu-Xin, WANG Xiao-Feng and TIAN Bo. A Study on Some Biochemical Characteristics of NuclearPolyhedrosis Virus of Ectropis grisescens warren[J]. Virologica Sinica, 1994, 9(3).

Citation: CAI Jian-He, HONG Yi-Guo, HUANG Fu-Xin, WANG Xiao-Feng, TIAN Bo. A Study on Some Biochemical Characteristics of Nuclear Polyhedrosis Virus of Ectropis grisescens warren .VIROLOGICA SINICA, 1994, 9(3) : 266.

中国南瓜曲叶双生病毒的生物学、血清学和分子杂交的研究

1.
广西农科院植保所中国科学院微生物研究所

摘要

从广西南宁市的南瓜上分离到一株可经烟粉虱（Ｂｅｍｉｓｉａｔａｂａｃｉ）传播的南瓜曲病毒，它可侵染南瓜（Ｃｕｃｕｒｂｉｔａｍｏｓｃｈａｔａ）和西葫芦（Ｃ．ｐｅｐｏ）引致叶片卷曲和严重矮化的症状。ＥＬＩＳＡ测定表明，其病叶粗提液与非洲木薯花叶病毒（ＡＣＭＶ－Ｋ）单克隆抗体ＳＣＲ１８呈强阳性反应，与ＳＣＲ１１呈较弱的阳性反应，而与ＳＣＲ２３呈阴性反应；与印度木薯花叶病毒（ＩＣＭＶ）单克隆抗体ＳＣＲ５６和ＳＣＲ６６呈强阳性巨应，与ＳＣＲ６２呈弱阳性反应，但与ＳＣＲ５２呈阴性反应。分子杂交试验进一步证实中国南瓜曲叶病的病原是南瓜曲叶双生病毒。
- 南瓜曲叶双生病毒生物学和血清学特性分子杂交

A Study on Some Biochemical Characteristics of Nuclear Polyhedrosis Virus of Ectropis grisescens warren

1.
Wuhan Institute of Virology,Academica Sinica,wuhan,430071

Abstract

In isolate of Chinese squash leaf curl virus(SqLCV-C),which is transmitted by whitefly Bemisia tabaci and caused leaf curl and severe stunting symptoms on Cucurbita moschata and C,pepo,have beenisolated and further confirmed by molecular hybridization using its coat protein gene probe in Nanningcity,Guangxi Province.ELISA tests indicated that this isolate had strong positive reactions with ACMV MAb-SCR18 and ICMV MAb-SCR56,-SCR66:mild reactions with ACMV MAb-SCR11 andICMV MAb-SCR62,but negative with ACMV MAb-SCR23 and ICMV MAb-SCR52.
- Chinese squash leaf curl geminivirus Biolugical and serological properties Molecular hy bridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Study on Some Biochemical Characteristics of Nuclear Polyhedrosis Virus of Ectropis grisescens warren

1. Wuhan Institute of Virology,Academica Sinica,wuhan,430071

Abstract: In isolate of Chinese squash leaf curl virus(SqLCV-C),which is transmitted by whitefly Bemisia tabaci and caused leaf curl and severe stunting symptoms on Cucurbita moschata and C,pepo,have beenisolated and further confirmed by molecular hybridization using its coat protein gene probe in Nanningcity,Guangxi Province.ELISA tests indicated that this isolate had strong positive reactions with ACMV MAb-SCR18 and ICMV MAb-SCR56,-SCR66:mild reactions with ACMV MAb-SCR11 andICMV MAb-SCR62,but negative with ACMV MAb-SCR23 and ICMV MAb-SCR52.