Use of Polymerase Chain Reaction for the Infection
with Herpes Simplex Virus in Chronic Cervicitis

CAI Gong; YAO Kun; JI Xiao-Hui; ZHOU Yao-Xi

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June 1994

CAI Gong, YAO Kun, JI Xiao-Hui and ZHOU Yao-Xi. Use of Polymerase Chain Reaction for the Infectionwith Herpes Simplex Virus in Chronic Cervicitis[J]. Virologica Sinica, 1994, 9(3).

Citation: CAI Gong, YAO Kun, JI Xiao-Hui, ZHOU Yao-Xi. Use of Polymerase Chain Reaction for the Infection with Herpes Simplex Virus in Chronic Cervicitis .VIROLOGICA SINICA, 1994, 9(3) : 272.

PCR在慢性宫颈炎HSV感染研究中的应用

1.
南京医科大学微生物学教研室

摘要

单纯疱疹病毒(HsV)被认为与宫颈癌有关。目前生殖道HSV感染日渐升高，日益引起人们的重视。由于药物治疗HSV感染有效，因而迫切需要一种简便、快速、有效的检测方法。以此为目的，我们用细胞裂解法结合FCR技术，检测了102份临床宫颈拭子中HSV—DNA，并与病毒分离、McAb-ELISA夹心法进行了比较，获得了满意结果，现报告如下。
- 多聚酶链反应单纯疱疹病毒宫颈炎

Use of Polymerase Chain Reaction for the Infection with Herpes Simplex Virus in Chronic Cervicitis

1.
Nanjing Medical University

Abstract

PCR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired methforwere allover 90%,It isshown that PCR cpmbined withcell lysis isa fast,effective and relle ble methed to detect HSV in the cervix,It can detect not only very low quantityHSV-DNA,but also inactive and latent HSV.HSV postivity rate of chronic cervicitis is markedly high-er than healthy control(P<0.05).
- Polymerase Chain Reaction Herpes Simplex Virus Cervicitis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Use of Polymerase Chain Reaction for the Infection with Herpes Simplex Virus in Chronic Cervicitis

1. Nanjing Medical University

Abstract: PCR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired methforwere allover 90%,It isshown that PCR cpmbined withcell lysis isa fast,effective and relle ble methed to detect HSV in the cervix,It can detect not only very low quantityHSV-DNA,but also inactive and latent HSV.HSV postivity rate of chronic cervicitis is markedly high-er than healthy control(P<0.05).