Isolation，Purification and DNA Polymerase Activity
Analysis of DI-IBV Repficatlve Complexe

SHAO Xin-Wu; DAO Pei-Zhen; GUO Ju-Chao; CHEN Hong-Shan

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June 1994

SHAO Xin-Wu, DAO Pei-Zhen, GUO Ju-Chao and CHEN Hong-Shan. Isolation，Purification and DNA Polymerase ActivityAnalysis of DI-IBV Repficatlve Complexe[J]. Virologica Sinica, 1994, 9(3).

Citation: SHAO Xin-Wu, DAO Pei-Zhen, GUO Ju-Chao, CHEN Hong-Shan. Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe .VIROLOGICA SINICA, 1994, 9(3) : 275.

鸭乙型肝炎病毒复制复合体（DHBV RCs）的提纯及其对外源模板的利用

1.
中国医学科学院医药生物技术研究所

摘要
- 鸭乙型病毒肝炎复合体
- , DNA聚合酶
- , 核心抗原
- , 外源模板

Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe

1.
Institute of Medical Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050

Abstract

Duck hepatitis B virus re plicativc complexe~ (DHBV RCs)wcl~e isol~ted from DI-~V—infected duck liver by chromato~aphy thxou Bio--Oel A 5m followed crose gradient centrifugation．The feIathe endogenous DNA polymcra．se activity of the purified RCs was 100 tim es higher than that of the unchromatosraphed one，confirmed by detecting their DHBcAg with Dot-EIA method．
- DHBV replicative complexes
- , DNA polymerase
- , DHBcAg．Exogenous template

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation，Purification and DNA Polymerase Activity Analysis of DI-IBV Repficatlve Complexe

1. Institute of Medical Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050

Abstract: Duck hepatitis B virus re plicativc complexe~ (DHBV RCs)wcl~e isol~ted from DI-~V—infected duck liver by chromato~aphy thxou Bio--Oel A 5m followed crose gradient centrifugation．The feIathe endogenous DNA polymcra．se activity of the purified RCs was 100 tim es higher than that of the unchromatosraphed one，confirmed by detecting their DHBcAg with Dot-EIA method．