我们从自然发病的大蒜中分离得到了大蒜花叶病毒。以其基因组ＲＮＡ为模板合成了３＇末端部分ｃＤＮＡ。从中选出一批插入片段在２．０ｋｂ以上的重组克隆，经Ｎｏｒｔｈｅｒｎ点杂交分析证实了所选克隆与基因组ＲＮＡ同源。通过对若干个克隆的插入片段两端部分序列的测定，选出一个克隆ｐＧＭ４９５，其插入片段的长度约为２．４ｋｂ，３′末端存有一个Ｐｏｌｙ（Ａ）结构，它应包含了编码该病毒外壳蛋白全部序列。序列测定的结果表明，这个ｃＤＮＡ片段全长为２３７９ｂｐ，其中含有与酶切图谱分析结果相符的ＥｅｏＲＩ、ＰｓｔＩ及ＢａｍＨＩ酶切位点。第一个终止密码子ＴＡＡ与３′ｇ末端相距２６４ｂｐ，我们根据碱基序列推定的氨基酸序列与其它已发表的Ｐｏｔｙｖｉｒｕｓ的外壳蛋白氨基酸序列以及外壳蛋白翻译后加工的蛋白酶专一切点相比较后推测，编码该病毒外壳蛋白序列可能起始于３′末端上游的１１７０ｂｐ处，共编码３０２个氨基酸，其分子量为３６ｋＤ，略大于ＳＤＳ－ＰＡＧＥ所测定的３３ｋＤ，非编码区域长２６４ｂｐ，富含ＡＴ，并有多个终止密码子的存在。趾３′末端３２～２７ｂｐ处有一个ＡＡＴＡＡ序列。

They have isolated garlic mosaic virus(GarMV)from naturally infected garlic plants and synthesized various pattial cDNA fragments using the GarMV genomic RNA as template.The length of thecoat protein(CP)gene was estimated about 0.8￣0.9 kb based on the estimated molecular weight ofthe coat protein subunit by protein SDS-Polyacrylamide gel analysis,Several clones containing cDNAfragments larger than 2kb were searched for a 3'terminal cDNA fragment including the complete CPgene and noncoding region.One clone,pGM495 containing a 2.4kb fragment was identified to be related to G arMv genomic RNA by Northern blotting assay and to contain the 3'-terminal poly(A)tract by terminal sequencing analysis suggesting the presence of these regions.The cDNA fragment in pGM495 has been completely sequenced.It contains 2379bp and three u-nique restriction sites,EcoRI I Pst I and BamH I ,which are conistant with the primary reetriction en-donuclease mapping analysis.There are several in-frame stop cedons,the first stop codon TAA is