Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species

WANG Wei; CA Yi-Quan; FANG Qin; LIU Yuan-Wen; KE Li-Hua

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August 1994

WANG Wei, CA Yi-Quan, FANG Qin, LIU Yuan-Wen and KE Li-Hua. Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species[J]. Virologica Sinica, 1994, 9(4).

Citation: WANG Wei, CA Yi-Quan, FANG Qin, LIU Yuan-Wen, KE Li-Hua. Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species .VIROLOGICA SINICA, 1994, 9(4) : 356.

草鱼出血病病毒多肽的基因定位

1.
中国科学院武汉病毒研究所

摘要

用聚丙烯酰胺凝胶电泳分离纯化的草鱼出血病病毒ＧＣＨＶ８７３的基因组ｄｓ－ＲＮＡ的１１个片段，分别在麦胚无细胞翻译体系中进行翻译。其翻译产物经ＳＤＳ－ＰＡＧＥ系统分析。结果表明，基因组片段１、２、３、４、５、和１０分别编码病毒核心衣壳的结构多肽ＶＰ１、ＶＰ２、ＶＰ３、ＶＰ４、ＶＰ５和ＶＰ１０，片段６和７分别编码病毒外层衣壳的结构多肽ＶＰ６和ＶＰ７。片段８和９分别编码５２ｋＤ和４１ｋＤ的多肽，片段１１编码两种多肽，分子量分别为２９ｋＤ和１９．５ｋＤ，它们与病毒结构多肽无明显对应关系。病毒基因组与多肽大体是一一对应的关系。
- 草鱼出血病病毒体外翻译基因定位

Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species

1.
Wuhan Institute of Virology,CAS

Abstract

ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation products were analysed in above system and l2 protein bands were displayed onthe X-ray film,RNA segments 1,2,3,4,5 and 10 coded individually for structural proteins of the in-ner virus shell,namely VP1,VP2,VP3,VP4,VP5 and VP10.RNA species coding for outer shell protenisVP6 and VP7 which are structural proteins were assigned to segments 6 and 7.Segments 8 and 9 codedindividually for VP52kD and VP 4l kD,and segment ll coded for VP29kD and VP 19.5kD.But weare not sure wether VP52kD,VP41kD,VP29kD and VP19.5kD are structural proteins or not.
- GCHV in vitro translation RNA-protein-coding assignment

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Gone-protein—coding Assignments of Grass Carp Hemorrhage Virus Genome RNA Species

1. Wuhan Institute of Virology,CAS

Abstract: ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation products were analysed in above system and l2 protein bands were displayed onthe X-ray film,RNA segments 1,2,3,4,5 and 10 coded individually for structural proteins of the in-ner virus shell,namely VP1,VP2,VP3,VP4,VP5 and VP10.RNA species coding for outer shell protenisVP6 and VP7 which are structural proteins were assigned to segments 6 and 7.Segments 8 and 9 codedindividually for VP52kD and VP 4l kD,and segment ll coded for VP29kD and VP 19.5kD.But weare not sure wether VP52kD,VP41kD,VP29kD and VP19.5kD are structural proteins or not.