The Preparation of the Fluorescence Antibody of Ovine Progressive Pneumonia Virus and the Detection of the Cell Infected by the Virus

DING En-Yu; XIANG Wen-Hua

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August 1994

DING En-Yu and XIANG Wen-Hua. The Preparation of the Fluorescence Antibody of Ovine Progressive Pneumonia Virus and the Detection of the Cell Infected by the Virus[J]. Virologica Sinica, 1994, 9(4).

Citation: DING En-Yu, XIANG Wen-Hua. The Preparation of the Fluorescence Antibody of Ovine Progressive Pneumonia Virus and the Detection of the Cell Infected by the Virus .VIROLOGICA SINICA, 1994, 9(4) : 375.

绵羊进行性肺炎病毒荧光抗体的制备及其对病毒感染细胞的检测

丁恩雨 ,
相文华

1.
复旦大学生命科学学院中国农业科学院哈尔滨兽医研究所

摘要

绵羊进行性肺炎（OPP）是慢性进行性传染病，以损害绵羊多种器官为特征，其病原为反转录病毒科慢病毒亚科的成员。目前回外对本病的研究．特别是有关诊断方面的报道甚多，而我国对OPP的研究尚处于起步阶段。本文旨在应用制备的OPP荧光抗体·按间接免疫荧光法(间接IFA)米检测OPPV感染绵羊胎肺细胞岳的变化情况，为进一步阐明OPPV及其它慢病毒的感染机理提供依据。
- 绵羊进行性肺炎病毒荧光抗体间接免疫荧光法

The Preparation of the Fluorescence Antibody of Ovine Progressive Pneumonia Virus and the Detection of the Cell Infected by the Virus

1.
College of Life Science,Fudan University

Abstract

The changes of sheep foetal lung cells infected by Ovine Progressive Pneumonia Virus(OPPV)were observed by.indirect immunofluorescence assay(IFA)and with the prepared fluorescence antibody of oppv.The result indicated that the fluorescence reaction initially appeared in 18 hours postinfection,The degree of the fluurescence reaction reached a high peak in 6 days postinfection and subsequently decreased.
- Ovine progressive pneumonia virus Fluorescence antiboody Indirect immunofluorescence assay

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Preparation of the Fluorescence Antibody of Ovine Progressive Pneumonia Virus and the Detection of the Cell Infected by the Virus

1. College of Life Science,Fudan University

Abstract: The changes of sheep foetal lung cells infected by Ovine Progressive Pneumonia Virus(OPPV)were observed by.indirect immunofluorescence assay(IFA)and with the prepared fluorescence antibody of oppv.The result indicated that the fluorescence reaction initially appeared in 18 hours postinfection,The degree of the fluurescence reaction reached a high peak in 6 days postinfection and subsequently decreased.