１９９３年４月从杭州蚕豆田病株上分离获得病毒分离物Ｂ－９３５Ａ，汁液摩擦接种５科１９种植物，Ｂ－９３５Ａ能侵染３科１２种植物。被侵染的蚕豆产生黄斑、花叶，后期叶片黄化并有坏死；在菜豆上产生局部枯斑。Ｂ－９３５Ａ的钝化温度为８５－９０℃，稀释限点为１０￣（－５）－１０￣（－７）病毒粒体杆状，平均长度约３００ｎｍ，病毒的衣壳蛋白亚基只有一条多肽链，分子量约为１７．５ｋＤ，包裹的核酸为申链ＲＮＡ，长约６．４ｋｂ．琼脂双扩散试验和免疫电镜试验表明，Ｂ－９２５Ａ与烟草花叶病毒（ＴＭＶ）有十分密切的血清学关系。根据以上试验结果，Ｂ－９３５Ａ被鉴定为烟草花叶病毒的蚕豆株系。这是国内外首次报道从蚕豆上分离到ＴＭＶ，也是我国首次从豆科作物上分离到的一个ＴＭＶ株系。

A virus isolate(B一935A)from faba 13e2．n w collected in Hangzhou．Zhejiang province．The isolate could infect 1 2 plant species in tkrce families． On faba bean，the isolate produced mosaic symptoms and necrosis．Its thermal inactivaflon point was 85— 90℃ ，dilution end po int was 1 0一一1 0 ．The virus particles wore rod—shaped with the length of 300nm ． The capsid protein of B一935A containod one po lypeptide with molecular weight of 1 7500．Th e viral genon~ consisted single strand RNA with the approximate length of 6．4kb． 1m muno-serological electron m icroscopy r ISEM ) and agro ge1 double diffusion test showed B一935A strongly reacted with the antisera of TMV． On the basis of these characteristics the B一935A w／Is identified lis a faba bean sCraM of TM V．This is the first report of TIVlV on faba bean and first repo rt ofTM V on legum inous cropsin China.