Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever

CHEN Yue; YANG Dong-Liang; HAO Lian-Jie; GAO Xue-Zhong; LIU Li-Kun

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April 1995

CHEN Yue, YANG Dong-Liang, HAO Lian-Jie, GAO Xue-Zhong and LIU Li-Kun. Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever[J]. Virologica Sinica, 1995, 10(2).

Citation: CHEN Yue, YANG Dong-Liang, HAO Lian-Jie, GAO Xue-Zhong, LIU Li-Kun. Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever .VIROLOGICA SINICA, 1995, 10(2) : 111.

流行性出血热循环免疫复合物组分分析

1.
同济医科大学附属同济医院临床免疫研究室
2.
湖北省郧阳地区人民医院
3.
安徽省阜阳地区出血热防治研究所

摘要

应用ＳＤＳ－聚内烯酰胺凝胶电泳及免疫转印技术对流行性出血热患者血清中免疫复合物组分进行了分析。流行性出血热循环免疫复合物经ＳＤＳ－ＰＡＧＥ分离，考马斯亮兰染色，显色主要有７条带，分子量分别为２３ｋＤ、５０ｋＤ、５２ｋＤ、６５ｋＤ、７２ｋＤ、８０ｋＤ及１００ｋＤ。采用该病毒特异性抗血清、单克隆抗体以及人免疫球蛋白、补体成分抗血清识别，在其循环免疫复合物中可检出特异性病毒抗原及相应的免疫球状蛋白和补体成分。应用ＰＣＲ技术在部分患者循环免疫复合物中可检出病毒核酸。上述结果从分子水平证实流行性出血热患者的ＣＩＣ中存在病毒抗原与特异性抗体是其循环免疫复合物的主要组分，它们构成了不同类型的免疫复合物，并且大多数可以结合补体成分。
- 流行性出血热
- , 循环免疫复合物
- , 抗原
- , 抗体
- , 补体

Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever

1.
Clinical Immunology Research Unil
2.
Tongji IIospital
3.
Tongji Medical University

Abstract

DS-polyacrylamide gel electrophoresis and Western blot analysis were carried out on the components of circulating immune complex(CIC) in sera of patients with epidemic hemorrhagic fever(EHF).After separtion in SDS-PAGE,the cumponents of CIC were stained with coomassie brilliantblue showing mainly 7 bands with appwrant molecular weight about 23kD,50kD,52kD,65kD,72kD,80kD and 100kD,respoctively.EHF virus antigens,human lg and the component of complement couldbe detected in the CIC using the speci fic antibod...
- Epidemic hemorrhagic fever
- , CIC
- , Antigen
- , Antibody
- , Complement

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever

1. Clinical Immunology Research Unil
2. Tongji IIospital
3. Tongji Medical University

Abstract: DS-polyacrylamide gel electrophoresis and Western blot analysis were carried out on the components of circulating immune complex(CIC) in sera of patients with epidemic hemorrhagic fever(EHF).After separtion in SDS-PAGE,the cumponents of CIC were stained with coomassie brilliantblue showing mainly 7 bands with appwrant molecular weight about 23kD,50kD,52kD,65kD,72kD,80kD and 100kD,respoctively.EHF virus antigens,human lg and the component of complement couldbe detected in the CIC using the speci fic antibod...