TIAN Xin, LI Jing-Yun, BAO Zuo-Yi, PAN Wen-Sheng and CHEN Wan-Rong. Establishment of a Sensitive ELISA for Detecting Antibodies against hepatitis E Virus Based on Synthetic Peptides[J]. Virologica Sinica, 1995, 10(2).
Citation:
TIAN Xin, LI Jing-Yun, BAO Zuo-Yi, PAN Wen-Sheng, CHEN Wan-Rong.
Establishment of a Sensitive ELISA for Detecting Antibodies against hepatitis E Virus Based on Synthetic Peptides .VIROLOGICA SINICA, 1995, 10(2)
: 115.
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摘要
用戊肝病毒(HEV)基因组编码氨基酸序列1-901-914/2-515-530、3-91-123、2-613-654相应的三段合成多肽为抗原,研制出一种检测抗-HEVIgG的ELISA试剂。以该试剂检测中国、缅甸、印度和前苏联肠道传播非甲非乙型肝炎(ET-NANBH)病人血清105份,仅3份中国病人血清阴性,阳性率为97.1%;检查实验感染HEV黑猩猩血清,感染前阴性,感染后阳性;检查正常人血清99份,全部阴性;检查甲肝(HA)、乙肝(HB)和丙肝(HC)病人血清46份,仅1份丙肝血清阳性(中和试验证明为抗-HEV真阳性)。和美国重组抗原抗-HEVELISA试剂比较,敏感性和特异性无显著差异,符合率为81.2%。用已知阳性血清8份,阴性血清2份作精确性试验,除1份阴性血清因OD值很低致变异系数偏高(33.5%)外,余变异系数均不超过10%。用已知阳性血清20份,阴性血清10份重复检测3次,平均OD值无显著性差异。上述结果表明,该抗-HEVELISA试剂具有很高的敏感性、特异性,检测结果稳定。
Establishment of a Sensitive ELISA for Detecting Antibodies against hepatitis E Virus Based on Synthetic Peptides
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Abstract
sensitive ELISA for detection of antibodies against hepatitis E virus(anti-HEV)was established,based on three synthetic peptides from open readin franmes ORF1,ORF2 and ORF3 of the HEVgenome.sera were tested for anti-HEV with our ELISA,One hundred and tWo(97.1%)out of 105sera from patients with enterically transmitted non-A non-B hepatitis E in Chil1a,Burma,India and former USSR were tested positive.A total of 99 sera from normal individuals in Beijing were tested negative.sera from experimentally infected c...
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