LI Gang, YAO Ji-Lu, PENG Wen-Wei, LV Ling and WANG Bin. Molecular Cloning and Sequence of the HCV NS1 Genomic Region[J]. Virologica Sinica, 1995, 10(2).
Citation: LI Gang, YAO Ji-Lu, PENG Wen-Wei, LV Ling, WANG Bin. Molecular Cloning and Sequence of the HCV NS1 Genomic Region .VIROLOGICA SINICA, 1995, 10(2) : 120.

HCV基因组NS1区的分子克隆及序列测定

  • 对广东省一名慢性丙型肝炎病人血清中的HCV基因组NS1区进行分子克隆及序列测定。采用微粒吸附法提取HCVRNA,随机引物逆转录后进行聚合酶链反应。所用引物位于NS1区,扩增产物780bp在低熔点琼脂糖中电泳,回收相应条带处凝胶,与pUC18的连接反应直接在低熔点琼脂糖中完成。重组体转化JM109,挑取菌落增殖后提取的质粒采用PCR和酶切法鉴定阳性克隆。将其中320bp的片段亚克隆pUC18和pUC19,随后采用双脱氧链末端终止法测其核苷酸序列,与国内外多个株比较,同源性介乎68.86%—90.84%。

Molecular Cloning and Sequence of the HCV NS1 Genomic Region

  • The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,w...

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    Molecular Cloning and Sequence of the HCV NS1 Genomic Region

    • 1. Department of Infectious Diseases
    • 2. Sun Yat-sen University of medical Sciences

    Abstract: The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,w...

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