XIANG Bing-Yi, YANG Yong-Feng, LIU Li-Bing, MA Wen-Yu, JIANG Chao-Zhun and SU Cheng-Zhi. Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV[J]. Virologica Sinica, 1995, 10(2).
Citation: XIANG Bing-Yi, YANG Yong-Feng, LIU Li-Bing, MA Wen-Yu, JIANG Chao-Zhun, SU Cheng-Zhi. Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV .VIROLOGICA SINICA, 1995, 10(2) : 131.

乙型脑炎病毒寡核苷酸探针的化学合成及初步检测

  • 根据已发表的乙型脑炎病毒核苷酸序列,设计合成了19寡核苷酸,作为检测该病毒的基因探针。以大肠杆菌DNA聚合酶IKlenow片段标记合成的寡核苷酸后,经过分子杂交证明该探针能检测出1pg靶DNA或JEV感染C6/36细胞6小时后的病毒。

Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV

  • probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.

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    Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV

    • 1. Institute of Molecular Biology
    • 2. The Fourth Military Medical University
    • 3. Xi an 710032

    Abstract: probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.

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