TANG Shi-Xing, WANG Hai-Tao, BAO Zuo-Yi and ZHANG Xi-Tan. A Skewed Light Chain Isotype Expression and B-Cell Clonal Dominance of Anti-HCV Response in Hepatitis C Virus Infection[J]. Virologica Sinica, 1995, 10(2).
Citation: TANG Shi-Xing, WANG Hai-Tao, BAO Zuo-Yi, ZHANG Xi-Tan. A Skewed Light Chain Isotype Expression and B-Cell Clonal Dominance of Anti-HCV Response in Hepatitis C Virus Infection .VIROLOGICA SINICA, 1995, 10(2) : 141.

HCV感染者抗HCV抗体产生的不均匀性与B细胞优势克隆

  • 本文采用抗原捕捉ELISA方法检测了HCV感染者血清中抗-HCVIgG抗体轻链κ和λ的比值,发现所检测的抗HCV-NS4、抗HCV-CP1和抗HCV-CP2抗体轻链的表达呈现明显的偏斜,65例抗HCV阳性者中63例(占96.9%),至少一种抗HCV抗体κ/λ偏离了正常1:1的比值,尤以λ链较多,分别占65.6%、89.9%和70.2%,但任何一个HCV感染者血清抗-HCV抗体既可能是κ链占优势,也可能是λ链为主;对6例抗HCV阳性者追踪观察了一年,发现抗HCV抗体κ/λ比值稳定不变。提示感染HCV后,病毒抗原可能稳定地刺激单个或少数B细胞优势克隆产生抗-HCV抗体现为HCV感染者抗-HCV抗体产生的不均匀性和B细胞优势克隆的存在,这一结果将有助于深入研究宿主抗HCV应答的规律,为抗HCV保护性免疫与HC疫苗研究提供线索。

A Skewed Light Chain Isotype Expression and B-Cell Clonal Dominance of Anti-HCV Response in Hepatitis C Virus Infection

  • In antigen capture ELISA in which HCV peptides NS4 CP1 and CP2 derived from Hcv NS4and core gene region,respectively,were used as solid-phase antigens was developed to detect the differences in light chain isotype expresslion of anti-HCV antibodies. Antibodies in 65 sera of HCV infectedprofessional blood donors against HCV NS4,CP1 and CP2 were characterized by a skewed light chainisotype expression. Sixty-three out of the 65 donors(96.9%)showed at least one of the three anti-HCV antibodies skewed from the no...

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    A Skewed Light Chain Isotype Expression and B-Cell Clonal Dominance of Anti-HCV Response in Hepatitis C Virus Infection

    • 1. Institute of microbiogy and Epidemiology

    Abstract: In antigen capture ELISA in which HCV peptides NS4 CP1 and CP2 derived from Hcv NS4and core gene region,respectively,were used as solid-phase antigens was developed to detect the differences in light chain isotype expresslion of anti-HCV antibodies. Antibodies in 65 sera of HCV infectedprofessional blood donors against HCV NS4,CP1 and CP2 were characterized by a skewed light chainisotype expression. Sixty-three out of the 65 donors(96.9%)showed at least one of the three anti-HCV antibodies skewed from the no...

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