Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

GONG Xiao-Meng; DU Nian-Xin; CAO Jia; CHEN Qing-Han; SONG De-Xiu

PDF
Cite
Share

facebook

twitter

google

linkedin

April 1995

GONG Xiao-Meng, DU Nian-Xin, CAO Jia, CHEN Qing-Han and SONG De-Xiu. Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity[J]. Virologica Sinica, 1995, 10(2).

Citation: GONG Xiao-Meng, DU Nian-Xin, CAO Jia, CHEN Qing-Han, SONG De-Xiu. Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity .VIROLOGICA SINICA, 1995, 10(2) : 177.

兔出血症病毒信使RNA的分离纯化及其生物活性的鉴定

1.
南京农业大学动物医学院
2.
中国科学院发育生物学研究所

摘要

用液相免疫沉淀法从感染兔出血症病毒（ＲａｂｂｌｔＨａｅｍｏｒｒｈａｇｉｃＤｉｓｅａｓｅＶｉｒｕｓ，ＲＨＤＶ）８小时后的兔肝组织的多聚核糖体中，分离出ＲＨＤＶ特异性的多聚核糖体，并由此获得全核酸，经Ｏｌｉｇｏ（ｄＴ）－ｃｅｌｌｕｌｏｓｅ亲和层析，得到３．４Ａ＿（２６０）的ＲＨＤＶｐｏｌｙ（Ａ）￣＋ＲＮＡ占全核酸的３．５％。此Ｐｏｌｙ（Ａ）￣＋ＲＮＡ经甲醛变性琼脂糖电泳，得到６条单一的带，其大小分别为０．９５、１．２２、１．８２、２．４０、３．６３和５．０１ｋｂ。在兔网织红细胞体外翻译体系中，此Ｐｏｌｙ（Ａ）￣＋ＲＮＡ能促进￣３Ｈ－亮氨酸的掺入，其掺入量为对照组的６．３倍。翻译产物经ＳＤＳ－ＰＡＧＥ和免疫转印可得到６条带，分子量分别为６４．５６、５８．８８、５３．７０、５１．４０、２８．４４和２３．９８ｋＤ。
- 兔出血症病毒
- , 信使RNA
- , 多聚核糖体
- , 免疫沉淀
- , 体外翻译

Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

1.
Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University
2.
Nanjing 210095Cao Jie

Abstract

Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...
- Rabbit haemorrhagic disease virus
- , Messenger RNA
- , Polysome immunoprecipitation
- , in vit ro translation

References
Proportional views

PDF

Article Metrics

Article views(3630) PDF downloads(876) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rabbit Haemorrhagic Disease Virus:Isolation of Messenger RNA and Identification of Its Activity

1. Department of Veterinary Microbiology and Immunology. Nanjing Agricultural.University
2. Nanjing 210095Cao Jie

Abstract: Poly(A)￣+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)￣+RNA was identified through denatural agarose elec-trophoresis Six single narrow bands,5.01,3.62,2.40,1.82,1.22 and 0.95 kb respectively,were vi-sualized.The in vitro translation products of viral mRNAs have been demonstrated by SDS-PAGE and immunoblotting analysis,proteins with ...