WANG Xi-Dong, CHEN Chen-Hua, YU Xiu-Beng, DONG Jie-De and CHOU Su-YIng. Studies on the Pathogenic M echanism of Viral M yocarditis—Assay of Lymphocyte Subsets and Anti—cardiac Antibodies[J]. Virologica Sinica, 1995, 10(3).
Citation: WANG Xi-Dong, CHEN Chen-Hua, YU Xiu-Beng, DONG Jie-De, CHOU Su-YIng. Studies on the Pathogenic M echanism of Viral M yocarditis —Assay of Lymphocyte Subsets and Anti—cardiac Antibodies .VIROLOGICA SINICA, 1995, 10(3) : 215.

病毒性心肌炎发病机理的实验研究──T细胞亚群和抗心肌抗体检测

  • 以柯萨奇B_3病毒(CVB_3)感染Balb/c小鼠建立心肌炎模型,检测了小鼠脾脏中T细胞亚群和血清中的抗心肌抗体,并进行了心脏病毒分离及组织病理学检查。结果显示,病毒感染后5天,脾脏中Thy1,2 ̄+(T_总)增高,7一15天降低,第21天恢复正常。L3T4 ̄+(Tc/Ts)于病毒感染后5一21天均明显高于对照组。L3T4+(Th/i)于病毒感染后7天开始增高,直到21天均明显高于对照组。病毒感染后15天,抗心脏肌球蛋白抗休开始增高,第21天继续增高。病毒感染后3一5天,心脏病毒分离均为阳性;第7天部分小鼠心脏中仍可分离出病毒;第15天心脏病毒分离为阴性。病毒感染后7一21天,心肌坏死、间质炎细胞浸润等病理变化进行性加重。上述结果提示,病毒感染本身和免疫因素都可能参与心肌炎的发病。

Studies on the Pathogenic M echanism of Viral M yocarditis —Assay of Lymphocyte Subsets and Anti—cardiac Antibodies

  • In this study,we established myocarditis model by using coxsackie type B3 inoculation.Results of the study revealed the following:5 days after inoculation,the percentage of Thylcells in the spleen of infected mice was increased,ttien it was decreased significantly on day7,15 and 2 1,L 3T4 ̄+subsets were increased on days 7,15and 2 1.Anti-cardiac antibodies inthe serum of infected mice were increased after inoculation.Virus was isolated from all thehearts of infected mice on days 3 and 5.The percentage of Dositive viral isolation were de-creased on day 7.Focal necrosis appeared in the myocardium with small lymphocytes infiltra-tion on day 5.Mvocardiac necrosis and inflammatory cell infiItration became more severe onday 7. and the ventricular cavity began to dilatate on days 1 5. Capillary and fibroblast in thenecrotic area appeared on day 2 1. The above results revcaled that T lymphocyte subsets,anti-cardiac antibodies and virus itself all may be associated with viral myocarditis.

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    Studies on the Pathogenic M echanism of Viral M yocarditis —Assay of Lymphocyte Subsets and Anti—cardiac Antibodies

    • 1. Shandong Medical University

    Abstract: In this study,we established myocarditis model by using coxsackie type B3 inoculation.Results of the study revealed the following:5 days after inoculation,the percentage of Thylcells in the spleen of infected mice was increased,ttien it was decreased significantly on day7,15 and 2 1,L 3T4 ̄+subsets were increased on days 7,15and 2 1.Anti-cardiac antibodies inthe serum of infected mice were increased after inoculation.Virus was isolated from all thehearts of infected mice on days 3 and 5.The percentage of Dositive viral isolation were de-creased on day 7.Focal necrosis appeared in the myocardium with small lymphocytes infiltra-tion on day 5.Mvocardiac necrosis and inflammatory cell infiItration became more severe onday 7. and the ventricular cavity began to dilatate on days 1 5. Capillary and fibroblast in thenecrotic area appeared on day 2 1. The above results revcaled that T lymphocyte subsets,anti-cardiac antibodies and virus itself all may be associated with viral myocarditis.

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