A Study on the Detection of Antibodies to HIV Type 1
and Type 2 by Using Synthetic Peptides

HONG Shi-Wen; HE Hong-Xia; SHU Feng-Lan; LIN Tao

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June 1995

HONG Shi-Wen, HE Hong-Xia, SHU Feng-Lan and LIN Tao. A Study on the Detection of Antibodies to HIV Type 1and Type 2 by Using Synthetic Peptides[J]. Virologica Sinica, 1995, 10(3).

Citation: HONG Shi-Wen, HE Hong-Xia, SHU Feng-Lan, LIN Tao. A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides .VIROLOGICA SINICA, 1995, 10(3) : 236.

人工合成多肽检测抗HIV－1和HIV－2抗体的研究

1.
中国人民解放军三０二医院

摘要

采用固相法合成ＨＩＶ－１和ＨＩＶ－２两个多肽，建立了用混合多肽为包被抗原检测ＨＩＶ－１和ＨＩＶ－２感染的间接酶联免疫吸附法。检测４６份抗ＨＩＶ－１和ＨＩＶ－２抗体阳性血清标本以及９４份对照血清标本，与ＵＢＩ试剂比较，其阳性符合率为９７．８％，阴性符合率为１００％，总符合率为９９．３％。实验结果表明，此法可用于ＨＩＶ－１和ＨＩＶ－２感染的检测。
- 合成多肽间接酶联免疫吸附法人免疫缺陷病毒1型人免疫缺陷病毒2型

A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides

1.
d hospital of PLA,Beijing 100039

Abstract

Two peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2.
- Synthetic peptide Indirect enzyme-linked immunosorbent assay Human immun-odeficiency virus type 1 Human immunodeficiency virus type 2

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptides

1. d hospital of PLA,Beijing 100039

Abstract: Two peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2.