Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana

XIAO Huo-Gen; GAO Qiao-Wan; ZHANG Shu-Guang; WANG Zhen-Zhong

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June 1995

XIAO Huo-Gen, GAO Qiao-Wan, ZHANG Shu-Guang and WANG Zhen-Zhong. Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana[J]. Virologica Sinica, 1995, 10(3).

Citation: XIAO Huo-Gen, GAO Qiao-Wan, ZHANG Shu-Guang, WANG Zhen-Zhong. Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana .VIROLOGICA SINICA, 1995, 10(3) : 254.

香蕉试管苗黄瓜花叶病毒检疫检验技术研究

1.
华南农业大学植物病毒研究室

摘要

建立了香蕉试管苗黄瓜花叶病毒ＤＡＣ－ＥＬＩＳＡ检测技术。应用０．１ｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ７．２，内含１％ＮａＤＩＥＣＡ）作为样品制备缓冲液，检测灵敏度可达到１／５１２０（稀释度）。两年来检测了１２８７个样品，香蕉试管苗生产繁殖材料的带毒率为２．８％。
- 香蕉试管苗黄瓜花叶病毒间接ELISA法

Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana

1.
Department of Plant Protection，South China Agricultural University, Guangzhou 510642

Abstract

The present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.
- Microprogation of banana Cucumber mosaic virus DAC-ELISA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana

1. Department of Plant Protection，South China Agricultural University, Guangzhou 510642

Abstract: The present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.