Purification and Specific Characterization of Potyvirus Helper Component
Abstract: Two helper components(HC)were purified with PEG precipitation and DEAE-cellulosechromatography from plants infected by tobacco etch virus(TEV)and watermelon mosaicvirus 2(WMMV-2).Bioassay results revealed that the Myzus persicae and Aphis gossypii cantransmit TEV after acquiring first TEV plus HC-TEV of HC-TEV,then TEV by parafilmmembrane method.HC encoded by WMMV-2 can assist aphids to transmit WMMV-2,butnot TEV,PAV-BYDV and tobacco mosaic virus(TMV).