The study on adaptation of Japanese encephalitits virus in Veto cells

DAN Hui-Ying; DING Zhi-Fen; CHANG Zhen-Yan

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August 1995

DAN Hui-Ying, DING Zhi-Fen and CHANG Zhen-Yan. The study on adaptation of Japanese encephalitits virus in Veto cells[J]. Virologica Sinica, 1995, 10(4).

Citation: DAN Hui-Ying, DING Zhi-Fen, CHANG Zhen-Yan. The study on adaptation of Japanese encephalitits virus in Veto cells .VIROLOGICA SINICA, 1995, 10(4) : 273.

流行性乙型脑炎病毒在Vero细胞上传代适应的研究

1.
北京生物制品研究所

摘要

通过乙脑病毒Ｐ＿３株以不同方式在鼠脑和Ｖｃｒｏ细胞间传代适应的研究，培育出适于工业化大生产制备乙脑疫苗的生产毒种。其毒力可达７．０±０．５ｌｇＬＤ５０／０．０４ｍｌ，空斑滴度为７．５±０．５ＰＦＵ／ｍｌ，免疫原性ＩＤ５０值为０．００００１６－０．００００２３ｍｌ；在含有适量人白蛋白的１９９营养液内，该毒种于－３０℃至少可稳定一年；与鼠脑组织毒种相比，传代细胞毒种的毒力、免疫原性及稳定性均与其相当。然而，后者不含脑组织，至少降低了一种过敏的危险。另外，细胞毒种的制备无需手工解剖鼠脑，易于控制外源因子污染，易于标准化。对于大规模生产疫苗、提高疫苗产量和质量，传代细胞毒种比鼠脑组织悬液毒种具有明显的优点。
- 乙型脑炎病毒传代适应 Vcro细胞

The study on adaptation of Japanese encephalitits virus in Veto cells

1.
Beijing Biological Product Institute

Abstract

3 strain of Japanese Encephalitis(JE)virus was adapted in Vero cells for 27 passages;Nosignlficant changes were found on virus titre and CPE after adaptation,but the immunogenicitywas getting weaker after 10 passages.The immunogenicity weakened could be recovered partlallyby repassag ing the V ero-cells adapted virus in mouse brain,Less than 10 passages of ad aptedviru s growing in Vero cells could be used as seed virus for prod uction of JE vaccine instead ofvirus prepared from mouse brain.U sing the ad apted virus for vaccine production could be easierto avoid the contamination of extraneous agents com pared with using the virus from mouse brain.
- Japanese Encephalitis Virus Adaptation of virus Vero cells

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The study on adaptation of Japanese encephalitits virus in Veto cells

1. Beijing Biological Product Institute

Abstract: 3 strain of Japanese Encephalitis(JE)virus was adapted in Vero cells for 27 passages;Nosignlficant changes were found on virus titre and CPE after adaptation,but the immunogenicitywas getting weaker after 10 passages.The immunogenicity weakened could be recovered partlallyby repassag ing the V ero-cells adapted virus in mouse brain,Less than 10 passages of ad aptedviru s growing in Vero cells could be used as seed virus for prod uction of JE vaccine instead ofvirus prepared from mouse brain.U sing the ad apted virus for vaccine production could be easierto avoid the contamination of extraneous agents com pared with using the virus from mouse brain.