用人重组肿瘤坏死因子－α（Ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ－α，ＴＮＦ－α）和人天然α干扰素（Ｉｎｔｅｒｆｅｒｏｎ－α－，ＩＦＮ－α）在人胚胎肺纤维母细胞（ＨＥＦ）和Ｈｅｐ－２细胞系上对常见呼吸道病毒所致细胞病变抑制进行比较观察。病毒包括不同型别的腺病毒５株，单疱病毒Ⅰ型（ＨＳＶ－Ｉ）１株，鼻病毒１株，仙台病毒１株，ＶＳＶ１株。结果提示ＴＮＦ－α和ＩＦＮ－α均具有广谱抗病毒活性。ＴＮＦ－α的抑毒作用能被ＴＮＦ－α申抗和ＩＦＮ－β单抗完全去除，被ＩＦＮ－α单抗部分去除ＴＮＦ－α的抗病毒效应。ＴＮＦ－－α中和试验的结果提示：ＴＮＦ抗病毒活性仍为ＩＦＮ－β诱生所介导。

This paper reports the protective effect of human rTNF-α and Human IFN-α on HEL andNEP-2 cell cultures infected by viruses in vitro,That is,before virus challenge,the treatment ofcell cultures in comparison with TNF-αand IFN-α may result in the inhibition of virus inducedcytopattiy.The challenge viruses included 5 strains of different tyDe of adenovirus,1 strain ofNSV-1,1 strain of rhinovirus,1 strain of Sendai virus,and 1 strain of VSVS,The antiviral ac-tivity of TNF-αand LFN-αare specific,and with wide spectrum.The specific antiviral activity ofboth TNF-αand IPN-α is confirmed by antibody neutralization. role of TNF-α inhibition can beneutralized completely by monoclonal IFN-β antibody and partly by monoclonal IFN-α antibody.The mechanism of antiviral effect of TNF-αis discussed. Aecording to the experiments ofneutralization test the TNF-α antiviral actlvity may be cumpletely neutralized by monoclonal anti-body againist IFN-β.It is strongly suggested that the antiviral effect of TNF-α appear to be me-diated