The Priliminary Study on Narcissus Infected with PVYG roup Viruses by Degenerate PrimerPCR Techniques

CHEN Zhi-Nan

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August 1995

CHEN Zhi-Nan. The Priliminary Study on Narcissus Infected with PVYG roup Viruses by Degenerate PrimerPCR Techniques[J]. Virologica Sinica, 1995, 10(4).

Citation: CHEN Zhi-Nan. The Priliminary Study on Narcissus Infected with PVYG roup Viruses by Degenerate PrimerPCR Techniques .VIROLOGICA SINICA, 1995, 10(4) : 346.

简并引物PCR技术研究洋水仙PVY组病毒初报

陈枝楠

1.
深圳动植物检疫局

摘要

利用简并引物ＲＴ－ＰＣＲ扩增技术，结合ＲＴ－ＰＣＲ扩增片段ＲＦＬＰ分析，对洋水仙１４个病毒病样品进行分组检测。结果表明洋水仙植物上存在有四种以上ＰＶＹ组病毒侵染。它们可能是ＴＢＶ、ＮＹＳＶ、ＮＳＳＶ和ＯｎＹＤＶ等。
- 洋水仙 PVY组病毒简并引物聚合酶链反应内切酶片段长度多态性

The Priliminary Study on Narcissus Infected with PVYG roup Viruses by Degenerate PrimerPCR Techniques

CHEN Zhi-Nan

1.
Shenzhen Animal and Plant Quarantme Service, shenzhen 518010

Abstract

More than four PVY group viruses were found to infect Narcissus by the techniques of de-generate primer reverse transcription(RT)-polymerase chain reaction(PCR)and restriction frag-ment length polymorphism(RFLP),they may be TBV,NYSV,NSSV and OnYDV etc.
- Narcissus PVY Group Degenerate primer RT-PCR RFLP

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Priliminary Study on Narcissus Infected with PVYG roup Viruses by Degenerate PrimerPCR Techniques

CHEN Zhi-Nan

1. Shenzhen Animal and Plant Quarantme Service, shenzhen 518010

Abstract: More than four PVY group viruses were found to infect Narcissus by the techniques of de-generate primer reverse transcription(RT)-polymerase chain reaction(PCR)and restriction frag-ment length polymorphism(RFLP),they may be TBV,NYSV,NSSV and OnYDV etc.