Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum

ZHANG Hai-Bao; ZHU Xi-Ru; ZHANG Yun-Kai

PDF
Cite
Share

facebook

twitter

google

linkedin

August 1995

ZHANG Hai-Bao, ZHU Xi-Ru and ZHANG Yun-Kai. Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum[J]. Virologica Sinica, 1995, 10(4).

Citation: ZHANG Hai-Bao, ZHU Xi-Ru, ZHANG Yun-Kai. Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum .VIROLOGICA SINICA, 1995, 10(4) : 367.

侵染菊花的黄瓜花叶病毒的初步鉴定和血清学检测

1.
中国科学院华南植物研究所

摘要

侵染菊花的黄瓜花叶病毒的初步鉴定和血清学检测张海保，朱西儒，张云开（中国科学院华南植物研究所广州５１０６５０）关键词菊花，黄瓜花叶病毒，间接ＥＬＩＳＡ菊花（Ｃｈｒａｙｓａｎｔｈｅｍｕｍｍｏｒｉｆｏｌｉｕｍ）病毒病是危害菊花的一类主要病害。国外文献报道...
- 菊花黄瓜花叶病毒间接ELISA

Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum

1.
China South Institute of Botang, Academia Sinica, Guangzhou 510650

Abstract

The virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The rate of transmission was 86.7%(13/15).The vlrus particle of isolate S_1 wassphere stiape, ItS diameter was about 28-30nm,The serological test showed that isolate S_1 re-acted positively with the antiserum against CMV but negatively with ToAV antiserum.On thebasis of these characteristics,isolate S1 was identified as a member of cucumber mosaic virus(CMV),The rabbit antiserum against CMV(isolate S1)was prepared with purified isolate S1.The highest titer of the antiserum was 1:2400 tested by ELISA.All 158 virus disease samples of field chrysanthemum plants were detected with the prepared antiserum by indirect-ELISA,inwhich 19.0% amples showed positive reaction.
- Chrysanthemum Cucumber mosaic virus（CMV） Indirect-ELISA

References
Proportional views

PDF

Article Metrics

Article views(3825) PDF downloads(780) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum

1. China South Institute of Botang, Academia Sinica, Guangzhou 510650

Abstract: The virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The rate of transmission was 86.7%(13/15).The vlrus particle of isolate S_1 wassphere stiape, ItS diameter was about 28-30nm,The serological test showed that isolate S_1 re-acted positively with the antiserum against CMV but negatively with ToAV antiserum.On thebasis of these characteristics,isolate S1 was identified as a member of cucumber mosaic virus(CMV),The rabbit antiserum against CMV(isolate S1)was prepared with purified isolate S1.The highest titer of the antiserum was 1:2400 tested by ELISA.All 158 virus disease samples of field chrysanthemum plants were detected with the prepared antiserum by indirect-ELISA,inwhich 19.0% amples showed positive reaction.