YANG Shou-Jing, LIU Yan-Fang, HU Ti-Qi, LI Ming-Sheng and HU Ming-Hua. Hemorrhagic fever with renal syndrome (HFRS) virus infection in the renal tissues of patients with HFRS studied by in situ hybridization and immunohistochemistry[J]. Virologica Sinica, 1996, 11(1).
Citation: YANG Shou-Jing, LIU Yan-Fang, HU Ti-Qi, LI Ming-Sheng, HU Ming-Hua. Hemorrhagic fever with renal syndrome (HFRS) virus infection in the renal tissues of patients with HFRS studied by in situ hybridization and immunohistochemistry .VIROLOGICA SINICA, 1996, 11(1) : 21.

用原位分子杂交和免疫组化研究肾综合征出血热肾组织中的病毒感染

  • 应用原位分子杂交和免疫组化技术研究了陕西、沈阳、上海、江西、广州等地收集的36例肾综合征出血热(HFRS)尸检肾组织中的病毒RNA和抗原的定位和分布。病毒RNA检测34例,28例阳性;抗原检测34例,30例阳性,病毒RNA及其抗原主要定位于细胞胞浆中。西安及沈阳地区的病例,病毒RNA和抗原阳性部位主要是肾小管上皮细胞,较少出现血管阳性。上海、江西和广州地区病例病毒RNA主要出现于肾间质血管和少数病例的个别肾小球毛细血管和肾小管上皮细胞;病毒抗原分布于肾间质血管和肾小管上皮中。1例(广州病例)肾血管平滑肌细胞检测到空泡状病毒NP抗原。另外在3例西安病例和9例上海病例肾远曲小管和集合管上皮中检测到NP和HA阳性的病毒包涵体。结果说明HFRS病毒可感染肾脏中肾小管上皮和血管内皮,并在肾小管上皮细胞产生包涵体。不同地区病例肾脏中病毒感染的部位不同,可能与不同地区病例所感染的病毒毒株或血清型不同有关。肾小管上皮细胞和血管内皮的损伤除病毒直接作用外,还可能有免疫因素参与。

Hemorrhagic fever with renal syndrome (HFRS) virus infection in the renal tissues of patients with HFRS studied by in situ hybridization and immunohistochemistry

  • emorrhagic fever with renal syndrome(HFRS)virus RNA and antigens were simultane-ously detected by in situ hybridization and immunohistochemical techniques in the autopsy renaltissues of the patients with HFRS.In 36 cases collected separately from Shaanxi,Shenyang,Shanghai and Jiangxi HFRS endemic foci,34 cases were subjected to virus RNA detection and 28cases were positive,34 cases were subjected to viral antigen detection and 30 cases were positive.Virus RNA and antigens were mainly localized in the cytoplasm,In the cases from Xi'an andShenyang,they were mainly distributed in the renal tubular cells.In the cases from Shanghai,Jiangxi and Guangzhou.virus RNA was mainly distributed in the renal interstitial vasculaturesand endothelial cells,occasionally in glomerular tufts and tubular cells,while viral antigens weredistributed in vasculatureb and renal tubular cells,and in Guangzhou cases in smooth muscles ofblood vessels。 Moreover,3 cases from Xi′an and 9 cases from Shanghai also displayed cytoplasmicinclusio

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    Hemorrhagic fever with renal syndrome (HFRS) virus infection in the renal tissues of patients with HFRS studied by in situ hybridization and immunohistochemistry

    • 1. Department of Pathology.Fourth Military Medical University,Xi'an 710032Department of Pathology,Shanghai Medical University,Shanghai 200032Department of Pathology.Jiangxi Medical College.Nanchang 320050

    Abstract: emorrhagic fever with renal syndrome(HFRS)virus RNA and antigens were simultane-ously detected by in situ hybridization and immunohistochemical techniques in the autopsy renaltissues of the patients with HFRS.In 36 cases collected separately from Shaanxi,Shenyang,Shanghai and Jiangxi HFRS endemic foci,34 cases were subjected to virus RNA detection and 28cases were positive,34 cases were subjected to viral antigen detection and 30 cases were positive.Virus RNA and antigens were mainly localized in the cytoplasm,In the cases from Xi'an andShenyang,they were mainly distributed in the renal tubular cells.In the cases from Shanghai,Jiangxi and Guangzhou.virus RNA was mainly distributed in the renal interstitial vasculaturesand endothelial cells,occasionally in glomerular tufts and tubular cells,while viral antigens weredistributed in vasculatureb and renal tubular cells,and in Guangzhou cases in smooth muscles ofblood vessels。 Moreover,3 cases from Xi′an and 9 cases from Shanghai also displayed cytoplasmicinclusio

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